Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Sugarcane Breeding Institute, Veerakeralam, Coimbatore-641007, Tamil Nadu (https://sugarcane.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: 1D and 2D gel electrophoresis unit and Iso-electric focusing unit
Make                                   : Bio Rad, GE Healthcare Life Sciences
Model                                  : Mini-PROTEAN® Tetra 1D electrophoresis (Bio-Rad, Laboratories, Inc., California, USA); Ettan DALT six electrophoresis unit (GE Healthcare Life ScienceBio-Sciences AB, USAUppsala, Sweden); Ettan IPGPhor II IEF unit (GE Healthcare Life ScienceBio-Sciences AB, USAUppsala, Sweden).
Specification   :Mini-PROTEAN® Tetra 1D electrophoresis (Bio-Rad, Laboratories, Inc., California, USA); Ettan DALT six electrophoresis unit (GE Healthcare Life ScienceBio-Sciences AB, USAUppsala, Sweden); Ettan IPGPhor II IEF unit (GE Healthcare Life ScienceBio-Sciences AB, USAUppsala, Sweden).

Click to ViewFrom 1D analysis to 2D gel differential spot detection with ImageMaster Platinum version 7 software (GE Healthcare)

  Working Principles
Click to ViewTwo-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a method of protein separation, by which proteins in a mixture are separated according to their isoelectric point (pI) in the horizontal direction (isoelectric focusing [IEF]) and molecular weight in the vertical direction (sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]). 2D-PAGE is used for the isolation/ separation/purification of proteins and further characterization with mass spectrometry and identification of specific proteins. The isoforms of a protein can easily be isolated with 2D- PAGE.

  Applications
  User Instructions
For profiling and separation of protein mixtures based on its molecular weight and isoelectric points. For detection of differentially abundant protein spots Extracted protein sample should be of high quality and the extraction buffer used for protein resuspension should be compatible with our downstream processes. We will not perform protein identification.

  Contact Us
Contact No. 0422 - 2472621
Email Id director@sugarcane.res.in

  1D and 2D gel electrophoresis unit and Iso-electric focusing unit Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Sophisticated Analytical Instrument (HPLC – Plant Pathology Lab) – Services- reg.
Rs. 7000/s
Rs. 7000/s
Rs. 7000/s

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, Sugarcane Breeding Institute, Coimbatore "
Letter, DD & Samples send to "The Director, Sugarcane Breeding Institute, Coimbatore "