Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500030, Talangana (www.icar-iior.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: 2D electrophoresis unit
Make                                   : Wipro GE
Model                                  : Ettan IPGphor 3 Isoelectric Focussing Unit
Specification   :

Click to View• current and voltage control of unit • Peltier cooling • Capable of running 1-12 IPG strps • temperature range: 15-25 deg C • Capable of running 7-24 cm IPG strps • Oil-free rehydration • Cy Dye protective lid • Monitor voltage, current and duration of the run • Copper electrode with gold plating

  Working Principles
Click to ViewEach protein in a cell has its characteristic isoelectric point and molecular weight. The combination of these two characteristic enable the separation of almost each of the proteins in biological sample loaded in a gel matrix. Separation of proteins extracted from a biological sample happens in first by their isoelectric point, the first dimension, by the application of high voltage in a very high resistance pH-gradient gel matrix. Upon the completion of the first dimension, the proteins are then separated based on their molecular weight in SDS-PAGE gel system and detection/visualization of the proteins is accomplished by Coomassie brilliant blue staining (sensitivity of  100 ng) or silver staining (sensitivity of  10 ng)

  Applications
  User Instructions
Separation of proteins for comparisons and detection of differentially expressed proteinsUsers need to specify the strips they plan to use. Preferably the isolated proteins must have been assessed for the quality as well as quantity using standard procedures so that the normalization with respect to protein samples to be separated using the 2D electrophoresis unit could be done. Also, enough protein samples must be available to run replicates. The procedure allows detecting the differentially expressed proteins but will not allow identification of the proteins.

  Contact Us
Contact No. 040-24598110
Email Id mulpuri.sujata@icar.gov.in

  2D electrophoresis unit Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Protein separation

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""