Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh (http://cpri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: 3500 Genetic Analyzer1
Make                                   : Hitachi Applied Biosystems
Model                                  : 3500 Genetic Analyzer 8 CH RUO
Specification   :

Click to View Capillary Length: 36 cm, 50 cm Compatible Polymers: POP-4™, POP-6™, POP-7™ Compatible Products: 3500 Genetic Analyzer Capillary Array (36 cm), 3500 Genetic Analyzer Capillary Array (50 cm), POP-4™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-6™ Polymer for 3500⁄3500xL Genetic Analyzers, POP-7™ Polymer for 3500⁄3500xL Genetic Analyzers Current Limit: 15 amps External Computer: Hard Drive: 2x 500GB SATA 3.0 Gb/s and 8 MB Data Burst Cache, Memory: 16 GB (2x 8GB) 1600 MHz DDR3 Non-ECC. 4th Gen Intel Core I7 Processor- 3.1 GHz Turbo Processor, Operating System: Windows 7 SP1 For Use With (Equipment): 3500 Genetic Analyzer Format: 8-well strips, 96-well plate Number of Capillaries: 8 Capillaries Operating Environment: Humidity: 20%-80% (non-condensing), Room temp: <2° fluctuation during runs, Temperature: 15°C-30°C Operating System: Windows 7 (Service Pack 1) Plan Duration: 1 Year Limited Warranty on Parts and Labor, Service Installation and Application Training Included Polymer Consumption: Replace at earliest of 7 days⁄384 samples⁄60 Injections (384-sample pouches), Replace at earliest of 7 days⁄960 samples⁄120 Injections (960-sample pouches) Product Size: 1 system Read Length: ≥850 bp using a 50 cm capillary and POP-7™ Throughput: Up to 424 samples per day (using run module HID36 POP-7) Voltage Limit: Electrophoresis Voltage – Up to 20 kV

  Working Principles
Click to ViewIn the theory, capillary electrophoresis occurs when an electric field (E) is applied to an electrolyte solution within a capillary, causing ions to migrates Driving force on ions is the applied electrical field to the electrolyte within a capillary DNA fragments are separated as they migrate by size exclusion Sieving effect Dye labeled fragments detected by fluorescence and laser and rendered into the sequenceIn the theory

  Applications
  User Instructions
DNA fragment Sequencing Fragment Analysis (Genetic fidelity test) Genotyping & Genomic Profiling Sanger Sequencing

  Contact Us
Contact No. 0177-2625073
Email Id director.cpri@icar.gov.in

  3500 Genetic Analyzer1 Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
sequencing analysis
4000
1500
1500
Per sample
fragment analysis per primer
2500
1000
1000
Per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR- Unit CPRI Shimla"
Letter, DD & Samples send to "The Director, ICAR-CPRI Shimla"