Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh (http://cpri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: 7900 HT Fast Real-Time PCR System
Make                                   : Applied Biosystems
Model                                  : 7900HT Fast Real-Time PCR System
Specification   :

Click to View Block Format:: Interchangeable Calibrated Dye:FAM™, JOE™, NED™, ROX™, SYBR® Green I, TAMRA™, TET™, VIC™ Capacity: Up to 36 x 96-well plates with optional automation accessory, Up to 84 x 384-well plates (with optional automation accessory and 384-well block) Detection Method: Primer-Probe Detection, SYBR Dimensions: 72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open) Dynamic Range: Up to 9 logs of linear dynamic range For Use With (Equipment): 7900 HT Fast System Format: 96-well plate High Throughput Compatibility: High Throughput-Compatible, Multiplexing Optics: Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm) Passive Reference Dye: No ROX, Other Passive Reference Dye, ROX (Pre-mixed), ROX (Separate Tube) Product Size: 1 instrument Reaction Speed: Fast Reaction Volume: 10-30 µl Run Time:33 minutes (96-well in fast mode) Sample Ramp Rate: Fast mode: ± 3.0°C⁄sec, Standard mode: ± 1.6°C⁄sec Sensitivity: Down to 10 copies Temperature Accuracy: ±0.25°C of display temperature Temperature Range (Metric):4-100°C Temperature Uniformity:±0.5°C Thermal Cycling System:Peltier-Based System Throughput:≥10,000 endpoint PCR rxns per hour (384-well block and automation accessory), ≥5,000 real-time PCR rxns per day (384-well block and automation accessory)

  Working Principles
Click to View The 7900 HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional fast real-time PCR capability. Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate. Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction. Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences. 96- or 384-well plate compatibility (including the TaqMan® Low Density Array). Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security. Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab’s productivity.

  Applications
  User Instructions
Real Time PCR-Based Gene Expression Profiling Copy number detection of genes qRT-PCR based detection of pathogen load in plant sample

  Contact Us
Contact No. 0177-2625073
Email Id director.cpri@icar.gov.in

  7900 HT Fast Real-Time PCR System Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""