Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh (http://cpri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: 96 wells StepOnePlusâ„¢ Real-Time PCR System
Make                                   : Applied Biosystems Inc, USA
Model                                  : StepOnePlusâ„¢ Real-Time PCR System
Specification   :

Click to ViewAbility to perform high-resolution melt analysis (additional software package required) Cost-effective 4-color/96-well system deliver precise, quantitative real-time PCR results Long-life, LED-based optical system records fluorescence from FAM™/SYBR® Green I, VIC™/JOE™, and ROX™ dyes for gene expression analysis, pathogen quantitation, SNP genotyping, and presence/absence assays. The StepOnePlus system also accommodates the TAMRA™ dye. The system perform Fast PCR reactions in less than 40 minutes Ultra-compact footprint fits any laboratory setting LCD touch screen and USB drive provide configuration flexibility and enable PC-free operation Remote monitoring and email notification for convenience and time savings The StepOnePlus system features VeriFlex Block technology, which combines six independently controllable Peltier blocks for enhanced PCR functionality and precise temperature control.

  Working Principles
Click to ViewSystem is equipped with fluorophore detection chemistries that include FAM and VIC dye–labeled Applied Biosystems™ TaqMan™ MGB probe-based assays, VIC and TAMRA dye–labeled probe-based assays, and SYBR Green I dye chemistry. [note: The TAMRA dye is available only with the StepOnePlus system.] TaqMan probe-based assays provide outstanding specificity and sensitivity, and SYBR Green I dye chemistry is an economical alternative for target identification, initial screening assays, or assays that require only a few reactions. The StepOnePlus system use fluorescent-based polymerase chain reaction (PCR) reagents to provide: Quantitative detection of target nucleic acid sequences (targets) using real-time analysis. Qualitative detection of targets using post-PCR (endpoint) analysis. Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs post-PCR). The system collect raw fluorescence data at different points during a PCR, depending on the type of run that the instruments perform.

  Applications
  User Instructions
Gene Expression Analysis MicroRNA Expression Protein Expression Translocation Analysis Gene Detection Viral Load Analysis SNP genotyping Translocation analysis

  Contact Us
Contact No. 0177-2625073
Email Id director.cpri@icar.gov.in

  96 wells StepOnePlusâ„¢ Real-Time PCR System Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
For testing of potato pathogen from leaves or tubers/Diagnostic uses
20,000
15,000
10,000
Minimum cost up to 5 samples
For quantification of gene expression
20,000
15,000
10,000
Minimum cost up to 5 samples
RNA/DNA extraction
8,000
6,000
4,000
Minimum cost up to 5 samples
Real time PCR analysis
12,000
9,000
6,000
Minimum cost up to 5 samples

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR- Unit CPRI Shimla"
Letter, DD & Samples send to "The Director, ICAR-CPRI Shimla"