Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Arid Zone Research Institute (CAZRI), Jodhpur 342003, Rajasthan (www.cazri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: AAS (Atomic Absorption Spectrophotometer)
Make                                   : Electronic Corporation of India Limited
Model                                  : AAS4141
Specification   :AAS without Graphite furnace

Click to ViewDouble-beam with Back-ground correction and Flame Emission Capability. Monochromator: Ebert design with 330mm focal length and 185-900 nm wavelength range. Automatic wavelength selection and peaking. Continuously adjustable slits with 02 to 2 nm spectral bandwidth. Manually operated turret holds four lamps. Individual adjustment for each lamp allows lamps to align to optical path. High intensity pulsed D2 lamp provides back ground correction. Auto setting of flame type and gas flow

  Working Principles
Click to ViewThe atoms in the non-excited, non-ionized, ground states are capable of absorbing light at characteristic wavelengths which they otherwise emit when excited. That makes the underlying principle of atomic absorption spectrophotometry. In the analysis employing Atomic Absorption Spectrophotometer (AAS), the sample in the form of a homogenous liquid is aspirated into a flame where “free” atoms of the element to be analyzed are created. A light source (hollow cathode lamp) is used to excite the free atoms formed in the flame by the absorption of the electromagnetic radiation. The decrease in energy (absorption) is then proportional to the number of free atoms in the ground state. The relationship between the amount of light absorbed and the concentration of the analyte present in known standards can be used to determine unknown concentrations by measuring the amount of light they absorb. An atomic absorption spectrometer is simply an instrument in which these basic principles are applied to practical quantitative analysis

  Applications
  User Instructions
Atomic absorption spectrometry (AAS) is an analytical technique that measures the concentrations of elements. Atomic absorption spectroscopy has various applications in various fields. One of the major applications of atomic absorption spectroscopy is for the determination of structural elucidation of various substances. Atomic absorption spectroscopy can be used to analyze water for its metal quantity if present. Atomic absorption spectroscopy is used in many pharmaceutical manufacturing processes where small quantities of catalyst remain in the final product. Atomic absorption spectroscopy can also be used in biological tissues such as blood, liver, brain tissue, muscle tissue and fluids for analyzing metals. In agriculture, it is used for the analysis of soils, plants, water and food materials etc. for measuring micronutrient contents. By using AAS the amount of metals present in rocks can be determined to see whether it is worth mining the rocks to extract the mineral.For extracting available micronutrients (DTPA extractable) in soil, weigh 10 g (2 mm sieved) soil in 100 ml conical flask. Add 20 ml of DTPA extractant [0.005 M DTPA + 0.1M Triethanolamine (TEA) + 0.01 M CaCl2, pH.7.3] and shake for 2 h on a mechanical shaker. Filter the content through Whatman No. 42 filter paper. It is better to discard first few drops for higher accuracy. The filtrate can be used for measuring the cationic micronutrients (Fe, Mn Zn and Cu) using AAS. In case of plant samples, digest 0.5 or 1 g oven dried sample in tri-acid mixture (AR grade conc. HNO3: H2SO4 : HClO4 (10:1:4) on hot-plate (temperature 180-200ï‚°C) or through microwave digestion system (do not use HClO4 in this system) and filter through Whatman No. 42 filter paper, make up the volume and take reading using AAS.

  Contact Us
Contact No. 0291-2788789
Email Id Praveen.Kumar@icar.gov.in

  AAS (Atomic Absorption Spectrophotometer) Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Standard Protocol of the nutrient/compound
1000/-
200/-

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, CAZRI, Jodhpur"
Letter, DD & Samples send to "Head Division of IFS ICAR – Central Arid Zone Research Institute Jodhpur - 342 003"