Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh (www.ivri.nic.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Agilent Microarray Hybridization Oven and scanner (SureScan)
Make                                   : Agilent
Model                                  : G2545A(Oven), G4900DA (Scanner)
Specification   :Oven:- Rotator Motor Speed - 2 to 20 RPM; Approximate Oven Dimensions (exterior) - Height: 22.0 in. (55.8 cm) Width: 17.4 in. (44.5 cm) Depth: 17.75 in. (39.5 cm); Approximate Chamber Dimensions (interior) - Height: 14.5 in. (36.8 cm) Width: 12.5 in. (31.8 cm) Depth: 12.0 in. (30.5 cm); Operating Temperature Range - Ambient + 5° to 70°C (+/- 0.1°C); Weight - 75 lbs (34.0 kg); Power Input - 110 – 120 Volts,Scanner:- Height - 42 cm (16.5 in); Width - 43 cm (17 in); Depth - 67 cm (26 in); Weight - 56.8 kg (125 lbs); Power Input - 100 – 240 Vac, 50 - 60 Hz, 250-VA max; Fuses - Two power supply fuses: T4A, 250 VAC (part# 2110-1491); Temperature - Operating: 15 ° to 30 °C, Storage: –40 ° to +50 °C; Humidity - Operating: 15% to 85% RH at 30 °C; Potentially sensitive to condensing humidity conditions; Altitude - Operating maximum: 4,600 m (15,000 ft), Storage maximum: 9,200 m (30,000 ft) at –40 °C; Laser Informations - Wavelengths: Green solid-state laser - 532 nm; Red solid-state laser: 640 nm; Power: both controlled to 13 mW . 1 mm smaller than scan region on the right, 2 mm on the left,

Click to ViewOven: Processes Agilent’s full range of microarrays, regardless of format Rotator rack holds up to 24 Agilent hybridization chambers Variable temperature control ranges from ambient + 5° – 70°C Variable rotation speed control Scanner: Dynamic autofocus Single and dual color scanning Automatic PMT gain calibration before each scan 2-, 3-, 5-, or 10-micron pixel size Dynamic range of >104 for a single scan in 16-bit scan mode, >105 for a single scan in 20-bit scan mode, and >106 for a dual scan in 16-bit scan mode (XDR) Uniformity specification of <5% CV (Coefficient of Variation) TIFF image file compression Flip and rotate images Internal and external barcode reading

  Working Principles
Click to ViewThe transcriptome is the complete set of transcripts in a cell, both in terms of type and quantity. Microarray is a technology to study the transcriptome of a population of cells, In this technology the control and experimental sample mRNA is transcribed into cDNA and labelled with CY3 and CY5 dyes,respectively. The labelled mRNA is allowed to hybridize on the microarray slide based on the principle that complementary sequences will bind to each other. The signal generated is read in the scanner and the feature extraction software converts the signal to quantitative terms.

  Applications
  User Instructions
Hybridization and ScanningSamples after the experiment/collection have to be immediately processed for RNA isolation or preserved in RNA Later. It should be borne in mind that the RNA quality is key to the success of the experiment

  Contact Us
Contact No. +91 581 2301584
Email Id ppgnbc@yahoo.co.in

  Agilent Microarray Hybridization Oven and scanner (SureScan) Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Hybridization and Scanning
Rs 25000/s
Rs 20000/s
Rs 20000/s
Charges are for per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit IVRI Izatnagar"
Letter, DD & Samples send to "Head Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar – 243122 , Bareilly, Uttar Pradesh "