Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Island Agricultural Research Institute, PB No. 181, Port Blair-744101, Andaman & Nicobar Islands (https://ciari.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Atomic Absorption Spectrophotmeter
Make                                   : SHIMADZU CORPORATION Analytical Division
Model                                  : AA-6200
Specification   :: Double Beam (chopper mirror) Monochromator: Abbration corrected Czerny-Turner monochromator; Holographic grating (1,600lines/mm) Wavelength range: 190 to 900 nm; Automated wavelength selection Slit: 0.2nm, 0.7nm Manual setting Background Correction: D2-Lamp method Lamp Turret: 2-lamps simultaneously lit (manual turret) Lamp Mode: Emission, Non-BGC, BGC-D2 Frequency:

Click to ViewAtomic absorption spectrometry (AAS) is an analytical technique that measures the concentrations of elements. Atomic absorption is so sensitive that it can measure down to parts per billion of a gram (µg dm–3) in a sample. The technique makes use of the wavelengths of light specifically absorbed by an element.

  Working Principles
Click to ViewAtomic absorption is the determination of the presence and concentrations of metals in liquid samples. Metals include Fe, Cu, Al, Pb, Ca, Zn, Cd and many more. Typical concentrations range in the low mg/L (ppm) range. In atomic absorption spectrometry, light of a specific wavelength is passed through the atomic vapor of an element of interest, and measurement is made of the decrease in the intensity of light as a result of absorption by atoms in the 'excited' state. The instrument is a piece of equipment that determines the concentration of a particular metal by focusing a beam of UV light at a specific avelength through a flame and into a detector. Metals will absorb ultraviolet light in their elemental form when they are excited by heat, either by flame or graphite furnace. Each metal has a characteristic wavelength that will be absorbed. The sample of interest is aspirated and atomized into the flame. If that metal is present in the sample, its atoms will absorb some of the light, thus reducing its intensity. This decrease in intesity of the light is the means by which the process of atomic absorption is established. The instrument measures the change in intensity. A computer data system converts this change into an absorbance.

  Applications
  User Instructions
One of the major applications of atomic absorption spectroscopy is for the determination of structural elucidation of various substances. This technique is more beneficial as a very small quantity of substance is required for analyzing. Atomic absorption spectroscopy can be used to analyze water for its metal quantity if present. Atomic absorption spectroscopy is used in many pharmaceutical manufacturing processes where small quantities of catalyst remain in the final product. Atomic absorption spectroscopy can also be used in biological tissues such as blood, liver, brain tissue, muscle tissue and fluids for analyzing metals. : 1.Press red power button. 2. Slide lamp into current source 2. Align the flame. 3. In the menu, choose “library”, then page down until “Fe-D2-248.3” is selected. Press button 2, then press enter. 4. Change the wavelength to 248.3 nm (that of the line source). Set the slit width (generally 2 Å = 0.2 nm). 5. Press “align”. Wait until instrument is done aligning. Press a/z. 6. To turn on the flame: a. Open the acetylene tank to ~12 psi. b. Plug in air compressor, switch knob to air. Turn up the fuel switch, adjust fuel. **when turning ON, start air before acetylene, to turn OFF, turn off acetylene before air.** 7. To take a reading: place clear tube in sample, press “read”. a. Watch the sample uptake closely. If you see a sudden change in signal, check to see whether any sample is entering the flame. If not, carefully adjust the knurled nebulizer adjustment. If you do this during an analysis, check the sample uptake rate. b. Wipe off the sample capillary tube with a Kim Wipe before transferring the tube to another container. 8. Use deionized water for the background. 9. At the end of the day: a. Close the gas tanks and purge the fuel and oxidant lines by opening the fuel and oxidant valves on the burner control unit until the regulator gauge reads 0. b. Run distilled water for several minutes before shutting down the flame.

  Contact Us
Contact No. 03192-250341
Email Id directorcaripb@gmail.com

  Atomic Absorption Spectrophotmeter Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Ca
165/- per
165/- per
165/- per
Manganese
110/- per
110/- per
110/- per
Iron
110/- per
110/- per
110/- per
Zinc
165/- per
162/- per
165/- per
Magnesium
110/- per
110/- per
110/- per
Molybdenum
330/- per
330/- per
330/- per

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, ICAR-CIARI, Port Blair"
Letter, DD & Samples send to "ICAR-Central Island Agricultural Research Institute Post Box No# 181 Port Blair -744 101 Andaman & Nicobar Islands"