Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), ICAR Campus, Ramagondanahalli, Yelahanka, Bengaluru-560064 Karnataka (www.nivedi.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Automated Microbial identification system
Make                                   : Applied biosystems, ThermoFisher scientific, USA
Model                                  : 3500 genetic analyzer with MicroSEQ® ID Microbial Identification Software
Specification   :

Click to View• The MicroSEQ® system comprises --> Bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries --> A fungal library containing more than 1100 entries • Radio frequency identification (RFID) technology tracks key consumables data and records administrative information • Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes • Provision of GeneMapper® software for fragment analysis • MicroSEQ® ID Microbial Identification Software --> Operating System: Windows 7, Windows Vista, Windows XP

  Working Principles
Click to ViewMicroSEQ® ID Microbial Identification Software allows easily identifying and classifying unidentified bacterial or fungal sequences by comparing them to a validated microbial library. This bundled version includes the bacterial and fungal sequence libraries comprising of over 2300 microbial 16S rDNA sequences.

  Applications
  User Instructions
• Identification of microbes based on rRNA gene sequence data • During analyzing the any project data, the library search report has to be evaluated to check the library matches against each specimen consensus sequence. Use the Library Search Report to: --> Identify the unknown organism. --> Assess the accuracy of the identification. --> Examine results for all matches. --> View phylogenetic relationships between the unknown and the top matches in a phylogenetic tree. --> Determine if a match was made against a validated (proprietary) library or a custom library. • To construct a phylogenetic tree, first search libraries to add sequences to tree and then search projects to add specimens to tree. • Click ‘Compute Tree’. The Tree Computation dialog box opens, then click OK. • Click the species name as desired in the tree, and then click the second species name as your second selection to view the Genetic Distance % for the selected species in the tree diagram • While viewing the tree, zoom the tree diagram as needed and select ‘File Print’ to print the tree diagram

  Contact Us
Contact No. 080-23093111
Email Id director.nivedi@icar.gov.in

  Automated Microbial identification system Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
2500
1000
2500

Additional Rs 100/-towards postal charges if result are to be posted
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