Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Sheep & Wool Research Institute, Avikanagar, via Jaipur-304501, Rajasthan (www.cswri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Automatic tissue processor and Stainer
Make                                   : Thermo Scientific
Model                                  : Citadel2000 & Varistain-24-4
Specification   :The compact design of the Citadel 2000 carousel tissue processor, coupled with its proven processing quality, ease of operation and excellent chemical resistance explains why it has become the tried and trusted choice for thousands of laboratories worldwide. The Varistain 24-4 is a versatile 24 position automatic staining instrument for processing microscope slides. It has been designed for simplicity and safety in operation and is intended for use in medical and research laboratories.

Click to ViewSpecimens are automatically raised from their reagent containers every 10 minutes and then reimmersed. Optional vacuum unit for efficient and rapid wax infiltration.Unique touch key pad incorporates all processing functions.Up to four complete schedules can be programmed.Programmable delayed start time. Loading and unloading of specimens is made easy with the specially designed tissue basket hanger. Random baskets holding 70 cassettes or 110 cassette organiser baskets. Smooth surfaces and contoured design facilitate easy cleaning. Integral fume hood greatly reduces release of vapours Easy change of reagent containers. This Shandon stainer features load-on-demand multiple baskets and programs, power outage backup and built in fume control to make staining easier, faster and safer. The Varistain 24-4 is a versatile 24 position automatic staining instrument for processing microscope slides. It has been designed for simplicity and safety in operation and is intended for use in medical and research laboratories. Flexible means of programming the unit enable it to be used for a wide variety of staining routines, using as few or as many of the 24 positions as are required, with periods of immersion at each stage from a few seconds upwards. More than one load of slides can be processed at the same time. Two identical 12 stage routines may be programmed for example, and continuous staining is also possible. During the staining programme an agitation facility can be switched on which caused the slides to be moved gently up and down in the reagents, increasing the staining efficiency and reducing the time required at each stage. At the end of the staining routine the slides remain immersed in the final trough until removed by the operator.

  Working Principles
Click to ViewWater is removed from the sample in successive stages by the use of increasing concentrations of alcohol. Xylene is used in the last dehydration phase instead of alcohol - this is because the wax used in the next stage is soluble in xylene where it is not in alcohol allowing wax to permeate (infiltrate) the specimen. This process is generally automated and done overnight. The wax infiltrated specimen is then transferred to an individual specimen embedding (usually metal) container. Finally, molten wax is introduced around the specimen in the container and cooled to solidification so as to embed it in the wax block. This process is needed to provide a properly oriented sample sturdy enough for obtaining a thin microtome section(s) for the slide. Once the wax embedded block is finished, sections will be cut from it and usually placed to float on a waterbath surface which spreads the section out. This is usually done by hand and is a skilled job (histotechnologist) with the lab personnel making choices about which parts of the specimen microtome wax ribbon to place on slides. A number of slides will usually be prepared from different levels throughout the block. After this the thin section mounted slide is stained with automatic stainer and a protective cover slip is mounted on it.

  Applications
  User Instructions
The histological slides are examined under a microscope by a pathologist, a medically qualified specialist who has completed a recognised training program. This medical diagnosis is formulated as a pathology report describing the histological findings and the opinion of the pathologist. The histopathology very useful in diagnosis of diseases. 1. When the tissue basket is raised, press to rotate the head to positions 2, 6,10, 2, 6 etc. 2. Positioning the head at any of these positions facilitates access to two groups of three reagent vessels and wax baths via the anti-evaporation covers. Stops the machine immediately. 3. A continuous ‘beep’ audible alarm sounds and the AMBER PROCESSING indicator flashes. If the machine is operating in Auto mode, it reverts to Manual mode. 4. Clears the alarm condition, providing that the cause of the alarm has been removed. Press once to silence the alarm then press again after a 3-second delay to restore the machine to its selected operating mode and program step. 5. If the alarm is caused by operator interference or a mechanical obstruction of the head, then, when you press the key the second time, the head moves to the nearest position and the tissue carrier lowers and raises. To resume operation, press the AUTO/MANUAL key.

  Contact Us
Contact No. 7568747105
Email Id sganesh413@gmail.com

  Automatic tissue processor and Stainer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Histopathology processing
150/tissue
50/tissue
50/tissue
The charges are only up to slide preparation, interpretation of slides required additional charges.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT -CSWRI, Avikanagar payable at SBI, MalpuraTonk Rajasthan"
Letter, DD & Samples send to "Head, Animal Health Division CSWRI, Avikanagar - 304501"