Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Research Institute for Jute & Allied Fibres, Barrackpore, Kolkata-700121 West Bengal (https://crijaf.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Biolog GEN III microbial identification system
Make                                   : Biolog, Inc.
Model                                  : BIOLOG MICROSTATION III
Specification   :GEN III Database, for both Gram-negative & Gram-positive bacteria. Includes appropriate Turbidity Standards

Click to ViewAnalyzes a microorganism in 94 phenotypic tests: 71 carbon source utilization assays and 23 chemical sensitivity assays. Provides a “Phenotypic Fingerprint” of the microorganism that can be used to identify it at the species level.

  Working Principles
Click to ViewAll necessary nutrients and biochemicals are prefilled and dried into the 96 wells of the MicroPlate. Tetrazolium redox dyes are used to colorimetrically indicate utilization of the carbon sources or resistance to inhibitory chemicals. Testing is performed very simply. The isolate to be identified is grown on agar medium and then suspended in a special “gelling” inoculating fluid (IF) at the recommended cell density. Then the cell suspension is inoculated into the GEN III MicroPlate, 100 μl per well, and the MicroPlate is incubated to allow the phenotypic fingerprint to form. All of the wells start out colorless when inoculated. During incubation there is increased respiration in the wells where cells can utilize a carbon source and/or grow. Increased respiration causes reduction of the tetrazolium redox dye, forming a purple color. Negative wells remain colorless, as does the negative control well (A-1) with no carbon source. There is also a positive control well (A-10) used as a reference for the chemical sensitivity assays in columns 10-12. After incubation, the phenotypic fingerprint of purple wells is compared to Biolog’s extensive species library. If a match is found, a species level identification of the isolate is made.

  Applications
  User Instructions
The GEN III MicroPlate™ test panel provides a standardized micro-method using 94 biochemical tests to profile and identify a broad range of Gram-negative and Gram-positive bacteria. Biolog’s Microbial Identification Systems software (e.g. OmniLog® Data Collection) is used to identify the bacterium from its phenotypic pattern in the GEN III MicroPlate.See Biolog’s Microbial Identification Systems software User Guide for further assistance in interpreting identification results.

  Contact Us
Contact No. 033-25356121(Extension No.209)
Email Id crijaf-wb@nic.in / director.crijaf@icar.gov.in

  Biolog GEN III microbial identification system Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT CRIJAF, BARRACKPORE"
Letter, DD & Samples send to "The Director, ICAR-CRIJAF, Barrackpore, North 24 Parganas, West Bengal 700120"