Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Agroforestry Research Institute, Jhansi, Near Pahuj Dam, Gwalior Road, Jhansi-284003 Uttar Pradesh (www.cafri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Biospectrometer
Make                                   : Eppendorf
Model                                  : BASIC
Specification   :Absorption measuring range 0 A – 3.0 A (260 nm) BSA concentration range 7.5 ng/µL – 45,000 ng/µL BSA concentration range (UV 280 nm) µCuvette G1.0 75 ng/µL – 45,000 ng/µL ±0.03 µg/µL at A = 0 ±0.076 µg/µL at A = 1 (0.5 %) BSA concentration range (UV 280 nm) UVette 10 mm 7.5 ng/µL – 4,500 ng/µL ±0.003 µg/µL at A = 0 ±0.007 µg/µL at A = 1 (0.5 %) BSA concentration range (UV 280 nm) UVette 2 mm 37.5 ng/µL – 22,500 ng/µL ±0.015 µg/µL at A = 0 ±0.038 µg/µL at A = 1 (0.5 %) Beam receiver fluorescence N/A Cuvette shaft 12.5 mm × 12.5 mm Detector Type CMOS photodiode array, 1024 pixel Emission wavelength I fluorescence – Emission wavelength II fluorescence – Excitation wavelength fluorescence – Fluorescence measuring range – Light path height 8.5 mm Light source absorption Xenon flash lamp Light source fluorescence – Measuring principle absorption Single-beam absorption spectrophotometer with reference beam Measuring principle fluorescence – Method memory >100 method programs Operator guidance language Spanish, Italian, French, English, German, Japanese Power consumption Approx. 15 W during operating step Approx. 5 W with dimmed display Random error absorption ≤0.002 at A = 0 ≤0.005 (0.5 %) at A = 1 Random error fluorescence – Random error wavelength ±1 nm Random temperature error – Scanning yes Smallest step size 1 nm Spectral bandwidth ≤4 nm Systematic error absorption ±1 % (A = 1) Systematic error wavelength ≤0.5 nm Systematic temperature error – Wavelength Selection Increment 1 nm Wavelength range 200 nm – 830 nm Wavelength range absorption Scan (nm): 200 – 830 at 1 nm increments dsDNA concentration range 2.5 ng/µL – 1,500 ng/µL dsDNA concentration range (UV 260 nm) UVette 10 mm 2.5 ng/µL – 150 ng/µL ±0.1 ng/µL at A = 0 ±0.25 ng/µL at A = 1 (0.5 %) dsDNA concentration range (UV 260 nm) UVette 2 mm 12.5 ng/µL – 750 ng/µL ±0.5 ng/µL at A = 0 ±1.25 ng/µL at A = 1 (0.5 %) dsDNA concentration range (UV 260 nm) µCuvette G1.0 25 ng/µL – 1,500 ng/µL ±1 ng/µL at A = 0 ±2.5 ng/µL at A = 1 (0.5 %) Interfaces › USB master: for USB stick and thermal printer DPU-S445 › USB slave for connecting to a PC (All functions available without PC) › Serial interface RS-232: for thermal printer DPU-414 › Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument Power supply 230 V, 50 – 60 Hz Dimensions (W × D × H) 295 × 400 × 150 mm / 11.6 × 15.7 × 6 in Weight w/o accessories 5.4 kg

Click to ViewThe biospectrometer basic is a small, very compact spectrophotometer that combines easy handling with highly reproducible analysis. It can measure and record UV/Vis spectral ranges as well as measure individual wavelengths from 200 nm to 830 nm. Its freely selectable wavelengths provide maximum flexibility for all current and future applications. In addition to preprogrammed methods for standard measurements, users can create and store their own methods. Optimized menu navigation guides through the individual methods in a step-by-step process. All required entries are visible, no essential data is omitted. A help box explains the operational sequences and is available in 5 different languages.

  Working Principles
Click to ViewUV spectroscopy obeys the Beer-Lambert law, which states that: when a beam of monochromatic light is passed through a solution of an absorbing substance, the rate of decrease of intensity of radiation with thickness of the absorbing solution is proportional to the incident radiation as well as the concentration of the solution. The expression of Beer-Lambert law is- A = log (I0/I) = Ecl Where, A = absorbance I0 = intensity of light incident upon sample cell I = intensity of light leaving sample cell C = molar concentration of solute L = length of sample cell (cm.) E = molar absorptivity From the Beer-Lambert law it is clear that greater the number of molecules capable of absorbing light of a given wavelength, the greater the extent of light absorption. This is the basic principle of UV spectroscopy.

  Applications
  User Instructions
 Nucleic acid quantification  Direct protein quantification (UV 280 nm)  Microvolume measurement via µCuvette G1.0 for highly concentrated samples  Bacteria growth measurement (OD 600)  Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry  Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)  Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytotoxicity assay The details viz., organism’s name, name of sample must be mentioned on a sample Nucleic acid and direct protein can be quantified The samples must be provide with appropriate package

  Contact Us
Contact No. 91-510-2730214
Email Id director.cafri@gmail.com

  Biospectrometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Absorbance method
500/ sampl
300/ sampl
300/sample
Depending upon the slot position the analysis will be done

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, ICAR-CAFRI, Jhansi"
Letter, DD & Samples send to "Director, ICAR-CENTRAL AGROFORESTRY RESEARCH INSTITUTE, GWALIOR ROAD, Jhansi-284003 (U.P.)"