Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh (www.ivri.nic.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Coonfocal Microscope
Make                                   : Olympus
Model                                  : IX81FV1000
Specification   :Upright Olympus IX81 microscope with motorized sectioning controlled with Fluoview acquisition software. Equipped with 4 lasers for fluorescence excitation (405nm, 488nm, 559nm and 635nm). 4 channel fluorescence detection with 2 spectral detectors and 2 photomultiplier tubes (PMTs) as well as a white-light channel PMT detector for differential interference contrast (DIC).

Click to ViewProcesses Agilent’s full range of microarrays, regardless of format Rotator rack holds up to 24 Agilent hybridization chambers Variable temperature control ranges from ambient + 5° – 70°C Variable rotation speed control

  Working Principles
Click to ViewConfocal microscopy, is an optical imaging technique for increasing optical resolution and contrast of a micrograph by using a spatial pinhole to block out-of-focus light in image formation.Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution.Light travels through the sample under a conventional microscope as far into the specimen as it can penetrate, while a confocal microscope only focuses a smaller beam of light at one narrow depth level at a time.Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures (a process known as optical sectioning) within an object. Imaging Options: z-stacks, multi-color, DIC, time-lapse, FRET, FRAP Sample options: fixed tissues and cells

  Applications
  User Instructions
3D reconstruction, time-lapse, FRET, FRAP and other photo-activation experiments. Ideal for imaging fixed tissue sections. Dye selection should be appropriate when using two or more dyes. The specimens, whether sectioned tissues or coverslip grown cultures, should be properly mounted before visualisation. Live cell culture visualisation facility is not available. To prevent the dye from bleaching off, avoid unnecessary exposure of the specimen/slide to the laser light source while working with the system

  Contact Us
Contact No. +91 581 2301584
Email Id ppgnbc@yahoo.co.in

  Coonfocal Microscope Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Confocal Microscopy
3500/- per
1800/- per
1800/- per
Rate are per hour basis

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit IVRI Izatnagar"
Letter, DD & Samples send to "Head Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar – 243122 , Bareilly, Uttar Pradesh "