Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), ICAR Campus, Ramagondanahalli, Yelahanka, Bengaluru-560064 Karnataka (www.nivedi.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Cryostat
Make                                   : Leica Microsystems, Germany
Model                                  : Leica CM1850UV
Specification   :

Click to View Sl. No. Description Leica 1850UV 1 Mechanism of cooling Peltier cooling, Single compressor 2 Disinfection Ultra violet 3 Cryobar temperature (specimen cooling area) 0 to -60 ºC 4 Motorised coarse advance 0.7mm/s 5 Section thickness adjustment 1 to 10µm in1µm increment 10 to 20µm in 2 µm increments 20 to 60µm in 5µm increments 6 Cryochamber temperature (Section cutting area) 0 to -35ºC 7 Low profile blades and high profile blades usage One holder is available to hold both blades but the high profile blades require pressure plate and it has to be fitted and unscrewed whenever required 8 Time required for cooling to -35ºC temperature 4 hours 9 Defrost cycle Automated 10 Touch screen Yes

  Working Principles
Click to ViewThe cryostat works in the principal that at low temperatures the liquid or the embedding medium freezes and acts as embedding material, the tissue becomes hard and becomes ice block. The microtome knife is used to make tissue sections of desired thickness without processing the tissues and fresh tissues can be used.

  Applications
  User Instructions
1. The Leica CM1850 is a powerful cryostat for routine as well as research applications in biology, medicine and industry. The instrument has been designed for rapid freezing and sectioning of tissue samples. 2. For use in Haematoxylin and Eosin staining of frozen tissue sections for early diagnosis 3. For use in immunohistochemistry staining of specific proteins/virus/bacteria/etc. 4. For use in cancer tissue sections to determine the prognosis immediately1. Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury. 2. Never leave knives and knife holders with a knife/blade mounted lying around 3. Do not place a knife on a table with the cutting edge facing upward 4. Always clamp the specimen before the knife 5. Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the hand wheel and cover the cutting edge with the knife guard 6. Avoid contact with cold parts of the instrument as this can cause frostbite 7. To make sure that the condensation water stemming from the defrost cycles drains into the waste container and to avoid the risk of possible contaminations, ensure that the tap of the waste container is open when operating the instrument. Only shut the tap when draining the waste container 8. It is not necessary to remove the microtome for routinely disinfecting the cryochamber 9. Only use the cleaning agents and disinfectants (alcohol or common disinfectants based on alcohol)

  Contact Us
Contact No. 080-23093110
Email Id director.nivedi@icar.gov.in

  Cryostat Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
1000
500
750

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""