Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Bureau of Animal Genetic Resources, P.B.No.129, G.T. Road Bye-Pass, Near Basant Vihar, Karnal-132001 Haryana (www.nbagr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: DNA Sequencer
Make                                   : ABI
Model                                  : 3100Xl Genetic Analyser
Specification   :ABI 3100Xl Genetic Analyser (DNA Sequencer) which is having 16 capillaries.

Click to ViewIt is sanger based sequencing.

  Working Principles
Click to ViewFor DNA sequencing..... 10 µl PCR product/clones should be cleaned and concentration should be 50 to 100 ng per µl 5 µl primer should also be provided at concentration of 5 p mole/µl Single pass sequencing would be done For Microsatellite genotyping..... Single/multiplexed PCR products with information on dyes used for tagging

  Applications
  User Instructions
DNA sequencing may be used to determine the sequence of genes, larger genetic regions (i.e. clusters of genes or operons), full chromosomes or entire genomes, of any organism.As per User manual

  Contact Us
Contact No. 9416296105
Email Id Madhu.Tantia@icar.gov.in

  DNA Sequencer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
1000/- per

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit NBAGR Karnal"
Letter, DD & Samples send to "Director, National Bureau of Animal Genetic Resources, GT Bye Pass Road, Karnal "