Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Research Centre on Meat, Chengicherla, P.B.No.19, Boduppal P.O., Hyderabad-500092, Telangana (www.nrcmeat.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Droplet digital (dd-PCR) PCR machine
Make                                   : Bio-Rad
Model                                  : QX-200
Specification   :Starting sample size, µl 20; QX200 droplet generator capacity 1–8 samples/cartridge; Droplets per 20 µl sample 20,000; QX200 droplet reader capacity 1–96 samples; Sample illumination Light-emitting diodes; Sample detection Multi-pixel photon counter; Detection channels FAM (EvaGreen), HEX (VIC); Linear dynamic range 5 orders of magnitude; Precision ±10%; Droplets per 96-well plate, million ~1.5

Click to ViewDroplet digital (dd-PCR) PCR machine including droplet generator, plate sealer, thermalcycler and droplet reader. The QX200 Droplet Digital PCR (ddPCRâ„¢) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

  Working Principles
Click to ViewIn dd-PCR, sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive measurement of nucleic acid amounts, compared to analogue PCR.

  Applications
  User Instructions
Amplification of DNA in rare events detection or mutation studies and when the copy number of DNA is suboptimal for analogue PCR.As per user manual

  Contact Us
Contact No. 040 29801672
Email Id nrcmeat_director@yahoo.co.in

  Droplet digital (dd-PCR) PCR machine Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Polymerase chain reaction
5000
5000
Per 96 well plate without consumables

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit, NRC on Meat"
Letter, DD & Samples send to "ICAR Unit, NRC on Meat payable at Hyderabad"