Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Citrus Research Institute, Post Box No.464, Shankar Nagar P.O., Nagpur-440 010 (www.ccringp.org.in)
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Instrument Details
Instrument/Facility Name: Electrophoresis with power pack
Make                                   : Hoefer inc.,
Model                                  : SUB25, PS 300B
Specification   :Maxi-plus standard submarine gel unit. Buffer chamber assembly, 1mm thick ,26 well combs -6 pcs, High voltage leads. Tray for casting gel. Hoefer power supply (10-300V,4-500mA)

Click to ViewMaxi-plus standard submarine gel unit. Buffer chamber assembly, 1mm thick ,26 well combs -6 pcs, High voltage leads. Tray for casting gel. Hoefer power supply (10-300V,4-500mA)

  Working Principles
Click to ViewElectrophoresis is a method used to separate charged particles from one another based on differences in their migration speed. In the course of electrophoresis, two electrodes (typically made of an inert metal, e.g. platinum) are immersed in two separate buffer chambers. The two chambers are not fully isolated from each other. Charged particles can migrate from one chamber to the other (Figure 1). By using an electric power supply, electric potential (E) is generated between the two electrodes. Due to the electric potential, electrons move by a wire between the two electrodes. More specifically, electrons move from the anode to the cathode. Hence, the anode will be positively charged, while the cathode will be negatively charged. As mentioned above, the two electrodes are immersed in two buffer chambers. Electrons driven to the cathode will leave the electrode and participate in a reduction reaction with water generating hydrogen gas and hydroxide ions. In the meantime, at the positive anode an oxidation reaction occurs. Electrons released from water molecules enter the electrode generating oxygen gas and free protons (which immediately form hydroxonium ions with water molecules). The amount of electrons leaving the cathode equals the amount of electrons entering the cathode. As mentioned, the two buffer chambers are interconnected such that charged particles can migrate between the two chambers. These particles are driven by the electric potential between the two electrodes. Negatively charged ions, called anions, move towards the positively charged anode, while positively charged ions, called cations, move towards the positively charged cathode.

  Applications
  User Instructions
Gel electrophoresis can use to separate DNA/RNA/ Protein fragment.Turn all power supply controls off and disconnect the power leads before removing the safety.

  Contact Us
Contact No. 0712- 2500249,2500813 Ext- 132
Email Id narakullav@gmail.com

  Electrophoresis with power pack Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

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