Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Flow Cytometer
Make                                   : Accuri
Model                                  : C6
Specification   :Laser Excitation : 488 nm and 640 nm. Laser Power : 14.7 mW 640 nm Diode Red Laser , 20 mW 488 nm Solid State Blue Laser Scatter detection : Forward (0 degrees, +/-13Side (90 degrees, +/-13) Emission Detection: 4 colors, standard optical filters Minimum Detectable Particle Size:0.5 µm Minimum Sample Volume:300 µL (in 12 x 75 mm tube) Data Acquisition Rate:10,000 events/second, maximum Typical Power Consumption :154 VA Heat Output:240 Btu/h Signal Processing :24-bit datapath Computer Interface:USB 2.0

Click to ViewThe cytometer is composed of fluidics, optics, and electronics subsystems that work together to analyze cell populations. Each particle passes through one or more beams of laser light. Scattered light and fluorescence emission provide information about the particle's properties. Information is gathered from the manner in which a particle scatters light or by the light emitted by fluorochromes attached to, or contained in, the particle. Light scattered in the forward direction of a laser beam is focused by a confocal lens and detected by a light detector which converts it into an electrical signal that is digitalised to generate a parameter known as Forward Scatter (FSC). The FSC signal will give information about the size and shape of the cell, and information can also be gathered by a side confocal lens and detected by a detector reading side scattered light. The Side Scatter (SSC) signal gives information about the granularity of the cell. As FSC and SSC are unique for each type of particle, the combination of the two can help identify different types of cells.

  Working Principles
Click to ViewFlow Cytometer uses sophisticated technology that makes use of the principles of light scattering by particles crossing a beam of light, and excitation and fluorescence emission of fluorochromes attached to specific molecules or expressed by cells, to identify, analyse, and/or sort different populations of cells

  Applications
  User Instructions
The ability of flow cytometers to analyse cells at high flow rate (up to 100,000 events per second) and to detect low signals make it possible to count cells and simultaneously analyse several cell physical and chemical properties with high sensitivity and in a very short period of time. Flow cytometry is also the technology of choice when it comes to purification of cells and separation of complex mixtures of cell populationsFollow the instructions as per the User manual. Prior confirmation may be obtained from the Institute before taking DD / sending samples.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  Flow Cytometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Flow Cytometry
10000
4500
5000
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod"
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"