Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), ICAR Campus, Ramagondanahalli, Yelahanka, Bengaluru-560064 Karnataka (www.nivedi.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Flow Cytometer
Make                                   : BD BioSciences, Belgium
Model                                  : FACS Cantoâ„¢ II system
Specification   :

Click to View• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT • Blue Laser Dyes: FITC, PE, PerCP or PerCP-Cy™5.5, PE-Cy™7 (525, 575, 678 or 695, 785 nm) • Red Laser Dyes: APC, APC-Cy7 (660, 785 nm) • Sample Injection: Direct into flow cell • Max Particle Size 50 μm • Sample Flow Rate, Min 10 μL/min • Sample Flow Rate, Max 120 μL/min • Sample Acquisition Rate: 10,000 events/second, 6 compensated fluorescence parameters and 2 scatter parameters • Sample Dead Volume: 30 μL (BD Falcon™ tubes 12 x 75-mm) • Integrated fluidics cart and compressor • Provision of fixed optical alignment procedure • The BD FACS™ Sample Prep Assistant II

  Working Principles
Click to ViewTCell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths. The fluorescence can then be measured to determine the amount and type of cells present in a sample. Applications :

  Applications
  User Instructions
• Cell counting, cell sorting, biomarker detection • Users can follow either of the standardize protocols as per the requirement of the user i.e. Direct staining, Indirect staining, intracellular staining or cell-cycle staining. • Direct staining: Live or fixed cells are incubated with directly labeled antibodies against cell surface antigens. • Indirect staining: If there is not a directly labeled antibody available, or user wish to amplify their signal he/she can do indirect staining. This is where user stain a cell with a primary antibody against the antigen of interest and visualize using a labeled secondary antibody which recognizes the primary. • Intracellular staining: In order to detect antigen not present on the cell surface, cells have to be fixed and permeabilized to disrupt the cell membrane and allow entry of the antibody. Antigens can be then directly or indirectly labeled. Various methods are optimal depending on the antigen and antibody used. • Cell-cycle staining: Measuring DNA content for cell cycle analysis requires fixation and permeabilization of the nuclear membrane. • Users can also follow the procedure for cell preparation, cell activation to determine immune competence, marker upregulation, cytokine release and proliferation by flow cytometry.

  Contact Us
Contact No. 080-23093111
Email Id director.nivedi@icar.gov.in

  Flow Cytometer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
2500
1000
2500

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""