Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
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Instrument Details
Instrument/Facility Name: GCMS
Make                                   : Agilent Technologies India Pvt. Ltd
Model                                  : GCMS 5975 Turbo system
Specification   :The Agilent 7890A Gas chromatograph utilizes 5th generation electronics pneumatic(EPC) control and digital electronics set a new benchmark for retention time locking(RTL) precision and help make the instrument most dependable. Faster oven cool down, robust backflush capability, advanced automation features and faster GC/MS oven ramps let you get more done in less time, at the lowest possible cost per sample. Powerful, chromatographer-friendly software simplifies method set up and system operation, and minimizes training time. Practical time saving design features speed up and simply routine maintenance.

Click to ViewGas Chromatography–Mass Spectrometry (GC-MS) is an analytical technique that combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within a test sample

  Working Principles
Click to ViewGas Chromatography–Mass Spectrometry (GC-MS) is an analytical technique that combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within a test sample. The difference in the chemical properties between different molecules in a mixture and their relative affinity for the stationary phase of the column promote separation of the molecules as the sample travels the length of the column. The heated gases are carried through a column with an inert gas (such as helium). GC is used to separate the volatile and thermally stable substitutes in a sample whereas GC-MS fragments the analyte to be identified on the basis of its mass. The molecules are retained by the column and then elute (come off) from the column at different times (called the retention time), and this allows the mass spectrometer downstream to capture, ionize, accelerate, deflect, and detect the ionized molecules separately. GCMS commonly uses electron impact (EI) and chemical ionization (CI) techniques. The main features like enhanced molecular ion, improved confidence in sample identification, significantly increased range of thermally labile and low volatility samples amenable for analysis, much faster analysis, improved sensitivity particularly for compounds that are hard to analyze allows GC- MS to use in broad range of areas.

  Applications
  User Instructions
It has broad range of applications that caters to academic research, quality control as well as industrial applications. GC-MS provides opportunity to analysis the new compounds for characterization and identification of synthesized or derivatives. GC-MS is exclusively used in monitoring and tracking organic pollutants in the environment. It can also be used to identify and quantify the various pesticides and its residues in agricultural products and other food commodities with high sensitivity. Likewise, chloro-phenols in water and soil, polycyclic aromatic hydrocarbons (PAH), organo-chlorine pesticides, herbicides, phenols, halogenated pesticides in analyte/samples can be conveniently screened by this technique.Agilent 7890A GC- Coupled with a triple quadrupole mass selective detector is used in electron impact (EI+) mode. The GC was equipped with a splitless injector at 250°C and a 30 m x 0.25 mm i.d., 0.25- µm HP-5ms column. The initial temperature was set at 50°C for 1 min, ramped at 25°C/min to 150°C, and ramped against at 10°C/min to 280°C hold for 4 min, with a constant helium flow of 1.3 ml/min. The mass selective detector temperatures were 280°C for both transfer line and source. Reference standards were optimized by Multiple Reaction Monitoring (MRM) method.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  GCMS Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
GC-MSpectrometry.
10000
5500
6000
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod."
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"