Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Research Centre for Grapes, PB No.3, Manjri Farm Post, Solapur Road, Pune-412307, Maharashtra (http://nrcgrapes.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Genetic Analyzer
Make                                   : Thermo Fisher
Model                                  : Applied Biosystem 3130
Specification   :4 capillary system and is being used for DNA fragment detection and sizing for accurate and reliable microsatellite analysis.

Click to ViewCapillary electrophoresis-Capillary electrophoresis offers several performance advantages for DNA analysis, compared with slab-gel techniques. The efficient heat dissipation of capillaries, along with a detection cell heater, provides enhanced thermal control, which results in more consistent runs and faster run times. Samples are simultaneously injected into the parallel 4- -capillary array in less than 30 seconds. Automated polymer delivery system-The System requires only sample preparation and creation of sample information for 24-hour continuous, unattended operation. Instrument set-up consists of filling the buffer reservoirs and attaching the polymer bottle. Flowable polymer is loaded into the capillaries prior to each run. Samples are injected and run according to conditions you specify. Data is collected and analyzed; the files are then available for direct transfer to a database for further analysis and reporting. Enhanced thermal control-The detection cell heater offers superior resolution and sizing precision, as well as improved thermal control. The new, higher temperature (60°C) 3130 POP-7™ Polymer run modules take advantage of the enhanced thermal control and improve run-to-run consistency. Detection method designed for sensitivity -The key to cost-effective and accurate nucleic acid analysis is uniform maximization of the amount of signal per sample. The detection system in the 3130 instruments is designed with this in mind. As the DNA passes through the detection cell, a laser beam simultaneously illuminates the capillaries from both sides of the array. The emitted fluorescent light is collected, separated by wavelength, and focused onto a charge coupled device (CCD). Spectral array detection-When the fluorescent light has been collected and dispersed across the CCD, the data are transferred to the instrument computer where they are transformed by chemometric algorithmic processing into 4- or 5-dye electropherograms.

  Working Principles
Click to ViewIt is a 4 capillary system of electrophoresis system and determines DNA fragment size with great accuracy. The DNA fragment is labelled with florescent dyes which are detected by a detector. Electrophoresis at very high voltage and presence of internal size standard in each sample ensures high resolution and accurate sizing.

  Applications
  User Instructions
Microsatellite analysis, AFLP analysis Only the instrument will be made available to the user. The user has to bring all the needed consumables like polymer, internal size standards etc. Assistance to analyze the samples and data analysis will be provided. Prior appointment to be taken to ensure the availability of instrument and personnel to run the equipment.

  Contact Us
Contact No. 7888000660
Email Id Anuradha.upadhyay@icar.gov.in

  Genetic Analyzer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Fragment analysis
5000
2000
2500

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""