Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Directorate of Groundnut Research, Ivnagar Road, P.B.No.5, Junagadh-362001 Gujarat (www.dgr.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: GenomeLab GeXP Genetic Analysis System
Make                                   : Beckman Coulter, Inc., USA
Model                                  : GeXP, S.N A220330260
Specification   :The Beckman Coulter GenomeLabâ„¢ GeXP Genetic Analysis System is a fully automated, versatile platform capable of a magnitude of genetic analysis applications ranging from quantitative multiplexed gene expression to fragment analysis and sequencing. The hardware is used to carry out the task of sample handling as well as the tasks associated with the separation and detection phases of electrophoresis. The software provides the user with an interface for manual or automatic control of the system, and with data capture and basic data analysis capabilities.

Click to ViewThe GenomeLab GeXP supports DNA sequencing and fragment analysis on one platform, with one capillary array, one gel and one software. This flexible system allows to run more than one application on the same plate and up to 192 samples. Uses linear polyacrylamide (LPA) – maximizing performance, coated eight-capillary array, four-wavelength laser-induced fluorescence detection, 96-well microplate format for samples/buffer, eight samples read in parallel, automatic gel replenishment, single setup facilitates gene expression analysis, DNA sequencing and fragment analysis

  Working Principles
Click to ViewThe GenomeLab Genetic Analysis System is fully automated and capable of determining the base sequence and fragment length of DNA samples prepared with Beckman Coulter, Inc. dye-labeled reagents. Four-color, dye-labeled terminator chemistry kits are used to process samples for base sequence analysis. Generation of samples for fragment length analysis is performed using either of two techniques: dye-labeled primers for micro-satellite, gene expression AFLP and other similar applications and four-color dye-labeled terminator chemistry, for the single nucleotide polymorphism application. Each sample, containing labeled DNA fragments, is automatically denatured and then separated by capillary electrophoresis. The separation gel is automatically replaced in the eight capillaries after each separation. The separation gel supply is an easily replaced cartridge with a capacity sufficient for two full microplates. The instrument performs detection using laser-induced fluorescence in four spectral channels. It automatically processes the four-channel raw data sets generated by each of the eight capillaries to produce high-quality base sequences or fragment lists after separation.

  Applications
  User Instructions
Gene Expression, Sequencing, SNP Analysis, STR Analysis, AFLP, MLPA (Multiplex Ligation-dependent Probe Amplification)Users are advised to submit Fluorescent or dye labelled (compatible with the instrument) PCR product of high quality with a minimum sample size of one plate (96 sample).

  Contact Us
Contact No. 0285-2673382 Ext 216
Email Id : chandramohan.sangh@icar.gov.in

  GenomeLab GeXP Genetic Analysis System Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Fragment analysis using GenomeLab GeXP Genetic Analysis System
3000
2100
2500
The cost mentioned is per sample and excluding GST

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Directorate of Groundnut Research, Junagadh OR Electronic transfer to ICAR-Directorate of Groundnut Research, Junagadh (ACC No: 10283132519 IFSC Code: SBIN0003251)"
Letter, DD & Samples send to "Director, ICAR-Directorate of Groundnut Research, PB 5, Junagadh, Gujarat, India-362001"