Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Marine Fisheries Research Institute, P.B.No.1603, Ernakulam North P.O., Abraham Madamakkal Road, Kochi-682018 Kerala (www.cmfri.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: High Performance Liquid Chromatography
Make                                   : Waters
Model                                  : Pico.Tag Waters
Specification   :High Performance Liquid Chromatography

Click to ViewA High Performance Liquid Chromatography (HPLC) is an amino acid analyzer that has ability in analyzing amino acids qualitatively and quantitatively. It inherits the features of high resolution and accurate compound detection with high sensitivity. HPLC system has UV detector system have with solvent delivery pump. Amino acid needs to be analysed according to Heinrikson and Meredith (1984) by digesting powdered diet samples (0.1 g) with 10 ml 6 N HCl at 110 deg C in sealed tubes for 24 h. Sample outputs are analysed using Breeze GPC software (Watersâ„¢, Milford, MA, USA).

  Working Principles
Click to ViewHigh performance liquid chromatography is a chromatography technique where the mobile phase is a liquid and the stationary phase is packed into a stainless steel column at high pressure. Sample containing a mixture of analytes is applied to a suitable chromatographic column and separation is achieved by pumping mobile phase through column. The differential interaction of analytes between stationary and mobile phase brings about separation. The concentration of the analyte is measured by detecting the amino acid as it elutes out of the column

  Applications
  User Instructions
A High Performance Liquid Chromatography is an amino acid analyzer that has a superior ability in analyzing amino acids qualitatively and quantitatively. It inherits the features of high resolution and accurate compound detection with high sensitivityExpected quantitative and qualitative data can be provided. The samples in processed conditions (please refer to the specifications, which detail the methodology of sample preparation) will only be accepted. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent should be given. The operating conditions should be supplied along with the sample. At present only RP C18 is available. You can submit the column of your interest for separation or repeatability of HPLC run. Source of the compound should be mentioned. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there is no toxic sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless accompanied by MS-DS. Only machine is available for use, client has to bring his/her own plastic ware etc, which are required. The facility is available during working hours of ICAR-CMFRI

  Contact Us
Contact No. 9496280584
Email Id vgcochin@hotmail.com

  High Performance Liquid Chromatography Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Amino acid composition
12500
7500
5000
Price per sample. Additional Rs 100/-towards postal charges if result are to be posted.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, ICAR-CMFRI, Cochin OR the payment can be made directly at ICAR-CMFRI office"
Letter, DD & Samples send to "The Director, ICAR-CMFRI, Ernakulam North PO, Kochi-682018, Kerala"