Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora-263601 Uttarakhand (www.vpkas.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: High Performance Liquid Chromatography at ICAR-VPKAS
Make                                   : Waters
Model                                  : 600E Controller/Pump, 717 Sampler, 9960 PDA
Specification   :A High Performance Liquid Chromatography (HPLC) is a analyzer that has a superior ability in analyzing organic compounds qualitatively and quantitatively. It inherits the features of high resolution and accurate compound detection with high sensitivity.

Click to ViewHPLC system have two detectors- Photo Diode Array detector (PDA) and Florescence Detector HPLC system have Waters 600E multi-solvent delivery pump

  Working Principles
Click to ViewHPLC (high performance liquid chromatography) is a chromatography technique where the mobile phase is a liquid and the stationary phase is packed into a stainless steel column at high pressure. It is usually silica particles mainly spherical nowadays. The efficiency is better when the particles are smaller typically 5µm. A pump is used to push the solvent through the column and a detector with a flow-through cell used to measure the separated peaks. Usually a computer with integration software collects the data and help quantifying the components. There are huge variations in the sample injection, pump, columns, detectors and software

  Applications
  User Instructions
A High Performance Liquid Chromatography (HPLC) is a analyzer that has a superior ability in analyzing organic compounds qualitatively and quantitatively. It inherits the features of high resolution and accurate compound detection with high sensitivity.1.Expected quantitative and qualitative data can be provided. 2.Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent should be given. 3.The operating conditions should be supplied along with the sample. 4.At present only RP C8,C18 are available. You can submit the column of your interest for separation or repeatability of HPLC run. 5.For HPLC PDA run frequency should be given. 6.Source of the compound should be mentioned. 7.MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there are no toxic sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless accompanied by MS-DS.

  Contact Us
Contact No. 05962-230208
Email Id Director.Vpkas@icar.gov.in

  High Performance Liquid Chromatography at ICAR-VPKAS Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Organic acid by HPLC
5000
5000
5000
per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, ICAR-VPKAS, Almora"
Letter, DD & Samples send to "Director, ICAR-VPKAS, Almora"