Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora-263601 Uttarakhand (www.vpkas.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: High Resolution Fluorescence Microscope
Make                                   : Olympus
Model                                  : BX 61
Specification   :The BX61 is specially designed to work with laser based autofocus unit. With precise focus and active tracking, users can speed up routine inspections.

Click to ViewOptical system: UIS2 optical system (infinity-corrected) Microscope frame: Illumination- Reflected/transmitted, External 12 V 100 W light source, Light preset switch, LED voltage indicator, Reflected/transmitted changeover switch. Focus-Motorized focusing, Stroke 25 mm, Minimum graduation 0.01 μm. Observation tubes: Widefield- Inverted: binocular, trinocular, tilting binocular; Erect: trinocular, tilting binocular. Super widefield- Inverted: trinocular; Erect: trinocular, tilting trinocular Reflected light illumination: BX-RLAA, Motorized BF/DF changeover, Motorized AS, Reflected fluorescence- BX-RFAA, Motorized 6 position turret Built-in motorized shutter with FS, AS Transmitted light: 100W halogen, Abbe/long working distance condensers Built-in transmitted light filters Revolving nosepieces: Motorized sextuple Stages: Coaxial left(right) handle stage: 76 (X) x 52 (Y) mm, with torque adjustment; Large-size coaxial left (right) handle stage: 10 0(X) x 105 (Y) mm, with lock mechanism in Y axis Dimensions: Approx. 318 (W) x 602 (D) x 541 (H) mm Weight: Approx. 25.5 kg (Microscope frame 11.4 kg)

  Working Principles
Click to ViewThe specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (xenon arc lamp or mercury-vapor lamp are common; more advanced forms are high-power LEDs and lasers), the excitation filter, the dichroic mirror (or dichroic beam splitter), and the emission filter. The filters and the dichroic beam splitter are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. In this manner, the distribution of a single fluorophore (color) is imaged at a time. Multi-color images of several types of fluorophores must be composed by combining several single-color images.

  Applications
  User Instructions
The user can identify the existence of even a minute scratch or flaw down to the 8 nm level--smaller than the resolving power limit of an optical microscope. It is also suitable for live cell imaging, high resolution microscopy, immuno- fluorescence, phenotypic assays and histology • As the microscope is a high precision instrument, always operate it with care, and avoid physical shake during the operation. • Do not let the microscope emerge in the sun directly, either not in the high temperature, damp, dusty or acute shake place. Make sure the worktable is horizontal. • When moving the microscope, use both hands to hold its hand-clasping and lay it down carefully • Fluorescence microscope should be used under dark environment. In order to protect eyes, do not stare at excitation light directly. • Fluorescence sample will be faded by ultraviolet radiation, so it can not for long save. Do not expose the sample under fluorescence light for long time, it will be quenched. • When working, the surface of lamp will be very hot. Make sure there is enough room for the heat dissipating around the lamp. • For safety, make sure the power switch is at "0" (off) and power it off before replacing the bulb or fuse, and wait until the lamp cools down. Wipe the lens gently with a soft lens tissue. Carefully wipe off oil or fingerprints on the lens surfaces with tissue moistened with a little of 3:7 mixture of alcohol and ether or dimethylbenzen. • Do not use organic solution to wipe the surface of the other components. Please use the neutral detergent. • If the microscope is damped by the liquid, cut off the power immediately and wipe it dry • Never disassemble or service the microscope yourself. It will influence its function or damage it. • After using, cover the microscope with a dust cover.

  Contact Us
Contact No. 05962-230208
Email Id director.vpkas@icar.gov.in

  High Resolution Fluorescence Microscope Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to "Director, ICAR-VPKAS, Almora"