Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute of Post-harvest Engineering & Technology, Post Office PAU, Behind Radha Swami Satsang Bhawan, Humbran Road, Ludhiana-141004 Punjab (www.ciphet.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: High pressure liquid chromatography
Make                                   : HITACHI
Model                                  : ELITE LaChrom-2000
Specification   :HPLC with C-8 and C-18 columns, diode array detector, auto-sampler, peristaltic pump and column oven; one point control software operated.

Click to ViewHPLC with C-8 and C-18 columns, diode array detector, auto-sampler, peristaltic pump and column oven; one point control software operated.

  Working Principles
Click to ViewChromatography employs a biphasic system: mobile and stationery phase to separate mixture of substances in to their components based on their molecular structure and composition. In HPLC, mobile phase is forced through a column under high pressure. The components of a mixture separate due to difference in the relative affinities of different molecules for mobile and stationery phases.

  Applications
  User Instructions
1. To analyze samples having minute quantity with accuracy 2. To identify, quantify and purify particular compounds 3. Has application in pharma, food, soil, residue testing and in clinics 1. Switch on HPLC and its software; make the pump on 2. Create a method; make necessary entries of the method and save it for use 3. Purge the system before operation for 15 min 4. Pour highly clear, membrane filtered (0.25/0.45 µm) samples in sample vials and place them in sample plate 5. Close the valve; select method and sample table and apply run 6. After completion of experiment(s), let the equipment run for 15 min 7. Finally switch-off pump and then software and equipment sequentially

  Contact Us
Contact No. 01634-224024
Email Id rajeshkumarciphet@gmail.com

  High pressure liquid chromatography Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Dr. Rajesh Kumar Vishwakarma, Principal Scientist
540
468
504
Per Sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""