Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Bureau of Agricultural Insect Resources, P.B.No.2491, H.A.Farm Post, Hebbal, Bellary Road, Bengaluru-560024, Karnataka (www.nbair.res.in)
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Instrument Details
Instrument/Facility Name: Horizontal Gel Electrophoresis Unit
Make                                   : Bio-Rad
Model                                  : Wide Mini-Sub Cell GT Cell
Specification   :

Click to View: The redesigned wide Mini-Sub cell GT electrophoresis cell offers updated features that make electrophoresis even easier. The wide Mini-Sub cell GT cell is suited for multiple-sample, rapid-screening applications. This popular system has a wide platform that can separate 30 samples per comb

  Working Principles
Click to ViewGel electrophoresis can be used to effectively separate nucleic acids based on their size within agarose or polyacrylamide gels. Size ladders containing a range of different-length DNA fragments are run with the sample of interest to identify the corresponding size bands in a gel. Agarose gels are ideal for the separation of DNA restriction digests, PCR products, and genomic DNA or RNA prior to Southern or northern blotting. Polyacrylamide gels can be used for what is known as polyacrylamide gel electrophoresis, or PAGE. Gels can be hand cast in the lab, or precast gels can be purchased from commercial provider

  Applications
  User Instructions
• Applications include oligonucleotide analysis, RNase protection assays, and northern blotting. Non-denaturing polyacrylamide gels are used to separate small double-stranded DNA (dsDNA) fragments, particularly PCR products. Denaturing polyacrylamide gels are used for separation of small RNA and single-stranded DNA (ssDNA) fragments. Good laboratory technique is essential when working with nucleic acids to minimize nuclease contamination. One critical consideration when working with nucleic acid is to avoid nucleases in your solutions, consumables, and labware.

  Contact Us
Contact No. 8023511998
Email Id directornbaii@gmail.com

  Horizontal Gel Electrophoresis Unit Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""