Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh (http://cpri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Ion Proton System for Next-Generation Sequencing
Make                                   : Ion torrent by Life technologies
Model                                  : Ion Protonâ„¢ Sequencer
Specification   :

Click to ViewCapacity: 1 chip per run Chip Compatibility: Ion PI™ Chip Comments: Compatible with Ion OneTouch™ automated template prep system Data Output: BAM Files, FASTQ Files, SFF Files, VCF Files Dimensions: Depth: 30.5 in (77.5 cm), Height: 18.7 in (47.4 cm), Width: 21.3 in (54.2 cm) For Use With (Equipment): Ion Proton™ System High Throughput Compatibility: High Throughput-Compatible, Multiplexing Multiplex Capability: DNA: 96 off-the-shelf barcodes, RNA: 16 off-the-shelf barcodes, Up to 384 barcodes Output:Ion Proton I Chip: Up to 2 human exomes per run Product Line: Ion Proton™, Ion Torrent™ Product Size:1 system Read Length: Up to 200 bp Reads⁄Run:Ion Proton I Chip: 60-80 million reads (passing filter at ≥50 bases) Run Time:2-4 hours Technique:Post-Light™ Ion Semiconductor Sequencing Throughput: Up to 10 Gb per run (Ion Proton I Chip) Weight: Crated for shipment: 200 lb (90.7 kg), Free-standing: 130 lb (59 kg)

  Working Principles
Click to ViewOn Proton™ System Exome WorkflowIon Proton™ System combines with the Ion TargetSeq™ Exome Kit and Ion Reporter™ Software to provide a simple and optimized exome workflow and two human-scale exomes per run. Ion TargetSeq™ Exome Kit provides up-to-date content, high coverage uniformity, and cost effective exome capture. Ion Reporter™ Software is a cloud-based solution that streamlines and simplifies analysis, annotation, and archiving of semiconductor sequencing data, and helps you interpret DNA variants faster. For Research Use Only. Not for use in diagnostic procedures. cpri Ion Proton™ System Whole Transcriptome WorkflowIon Proton™ System combines with the Ion Total RNA-Seq Kit v2, Ribominus™ Eukaryote Kit v2 and third party software packages such as Partek® and Avadis® to provide a simple and optimized whole transcriptome workflow. Ion Total RNA-Seq Kit v2 provides a fast, simple workflow with as little as 100 ng of total RNA input, while maintaining strand orientation and minimizing bias and error. Ribominus™ Eukaryote Kit v2 provides more than 90% rRNA depletion increasing the sequencing power of the Ion Proton™ System. Third party software package such as those provided by Partek® and Avadis® minimize the data analysis time by providing comprehensive tools and auto-analysis direct from the Ion Proton™ System.Partek is a registered trademark of Partek Inc. Avadis is a registered trademark of Strand Life Sciences Private Limited. For Research Use Only. Not for use in diagnostic procedures. cpri Ion Proton™ System Targeted Sequencing WorkflowIon Proton™ System* combines with the Ion AmpliSeq™ target selection technology and Ion Reporter™ Software to provide a simple and optimized targeted sequencing workflow. Ion AmpliSeq™ technology offers both custom panels and ready-to-use panels for flexible and simple targeted sequencing of genes or genomic regions. Ion Reporter™ Software is a cloud-based solution that streamlines and simplifies analysis, annotation, and archiving of semiconductor sequencing data, and helps you interpret DNA variants faster.*The content herein may relate to products that have not been officially released and is subject to change without notice for research use only. Not for use in diagnostic procedures. cpri

  Applications
  User Instructions
DNA Sequencing of microbial pathogens Epigenetic Sequencing of plant varieties Fragment Analysis Genotyping & Meta Genomic Profiling RNA Sequencing and small RNA profiling

  Contact Us
Contact No. 0177-2625073
Email Id director.cpri@icar.gov.in

  Ion Proton System for Next-Generation Sequencing Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
3.50 Lac
2.50 Lac
2.50 Lac
Per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR- Unit CPRI Shimla"
Letter, DD & Samples send to "The Director, ICAR-CPRI Shimla"