Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Spices Research, Marikunnu P.O., Kozhikode-673012, Kerala (www.spices.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Iso Electric Focusing system
Make                                   : GE Health care
Model                                  : EttanIPGPhor 3
Specification   :

Click to ViewIso electro focusing, is a technique for separating proteins or peptides, on the basis of their isoelectric point

  Working Principles
Click to ViewSeparation is achieved by initially establishing a pH gradient along the gel using a mixture of low-Mr carrier ampholytes such as amino acids or small peptides. The sample is then move through the pH gradient under an electric field until their individual charges reach zero, i.e., when they attain the pH corresponding to their isoelectric point. The high resolving power allows the separation of compounds differing by only 0.01 pH units in pI.

  Applications
  User Instructions
Used as first stage separation for 2D-PAGE. Fractionation of proteins or peptides prior to mass spec. More peptide identifications from whole proteome samples than other methods of protein separation. Available with the contact person

  Contact Us
Contact No. 0495-2731410
Email Id umadevi@spices.res.in

  Iso Electric Focusing system Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
8000
5000
6000
charges are per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit-IISR, Calicut"
Letter, DD & Samples send to "Head, SAIF, ICAR-Indian Institute of Spices Research, Marikunnu P.O., Kozhikode - 673012, Kerala."