Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora-263601 Uttarakhand (www.vpkas.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Kjeltec Auto analyser
Make                                   : FOSS
Model                                  : FOSS Kjeltec -2300
Specification   :Kjeltec-2300 is a standalone unit for automatic determination of nitrogen, according to kjeldahl method

Click to ViewThe steps includes dilution of digested samples, reagent addition, distillation, titration waste disposal. Calculation of Total Nitrogen content of samples.

  Working Principles
Click to ViewThe Kjeldahl method or Kjeldahl digestion in analytical chemistry is a method for the quantitative determination of nitrogen contained in organic substances plus the nitrogen in inorganic ammonia and ammonium (NH3/NH4+). Other forms of inorganic nitrogen, for instance nitrate, are not included in this measurement. This method was developed by Johan Kjeldahl in 1883. The method consists of three basic steps: 1) digestion of the sample in sulfuric acid with a catalyst, which results in conversion of nitrogen to ammonia; 2) distillation of the ammonia into a trapping solution; and 3) quantification of the ammonia by titration with a standard solution. During digestion with H2SO4 organic nitrogen is converted to NH4+-N.K2SO4 increases the temperature of digestion, Cu and Se react as catalysts to increases the rate of oxidation of organic matter by H2SO4. TheNH4+-N in the digest is determined by collection of NH3 liberated by distillation of the digest with alkali. The distillate is analyzed for NH4-N by titration. Compounds containing N-N and N-O linkages (e.g., nitrites, nitrates, nitroso and nitro compounds) are not included when determining total nitrogen.

  Applications
  User Instructions
Quantitative estimation of total/available nitrogen in soil/ plants/ compost/ leaf litters etc and crude protein content in seedsQualitative and quantitative data can be provided, All sample should be homogenous. Samples should not be toxic or hazardous.

  Contact Us
Contact No. 05962-230208
Email Id Director.Vpkas@icar.gov.in

  Kjeltec Auto analyser Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Available Nitrogen
500
500
500
per sample
Total Nitrogen
500
500
500
per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "Director, ICAR-VPKAS, Almora"
Letter, DD & Samples send to "Director, ICAR-VPKAS, Almora"