Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute of Fisheries Technology, CIFT Junction Matsyapuri PO, Cochin-682029, Kerala (www.cift.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Microarray Scanner
Make                                   : Agilent, USA
Model                                  : SureScan Microarray Scanner (G2600D), 2013
Specification   :Microarray Scanner is part of the microarray analysis solution from Agilent Technologies. Sophisticated laser-induced fluorescence scanner designed to read microarrays printed on standard 1 in × 3 in slides.

Click to View(a) G2600D SureScan scanner with integral 24-slide cassette (b) 24 slide holders (c) Computer workstation with recovery software (d) Microarray Scan Control Software with other accessories (e) Dynamic autofocus (f) Single and dual color scanning (g) Automatic PMT gain calibration before each scan (h) 2-, 3-, 5-, or 10-micron pixel size (i) Dynamic range of >104 for a single scan in 16-bit scan mode, >105 for a single scan in 20-bit scan mode, and >106 for a dual scan in 16-bit scan mode (XDR) (j) Uniformity specification of <5% CV (Coefficient of Variation)

  Working Principles
Click to ViewMicroarray scanner measures the fluorescence intensity of labeled sample nucleic acid (DNA and RNA) bound to microarrays. Its ability to measure fluorescence from two dyes simultaneously facilitates all two-color microarray studies. This technology provides for rapid, high-quality, automated scanning of microarrays. Probe spotted onto the slide will be hybridized with the nucleic acid from the sample and fluorescence detected with the sure scanner software provided with the system. The SureScan scanner uses two lasers; a green diode-pumped solid-state laser (532 nm) and a red diode laser (640 nm). The lasers excite Cyanine-3 (Cy-3) and Cyanine-5 (Cy-5) labeled RNA or DNA to measure fluorescence after hybridization of the target nucleic acid to the microarray probes. Dyes similar to Cy-3 and Cy-5, such as Alexa 647, 555, and 660 dyes, can also be used. The SureScan scanner is optimized for high signal-to-noise performance in the Cy-3 (550 — 610 nm) and Cy-5 (650 — 750 nm) emission bands.

  Applications
  User Instructions
Sample should be submitted in as DNA or RNA or PCR amplified and purified products either in solid or solution form. Testing available for WSSV, IHHNV, MBV and HPV from Shrimp samples. Should be supplied in microgram per microlitre concentration. Should be free from contaminating protein or nucleic acid. Should be supplied in chilled containers.

  Contact Us
Contact No. 0484-2412300
Email Id prasadmm@hotmail.com

  Microarray Scanner Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
25000
2500
7500
per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, ICAR-CIFT, Cochin OR the payment can be made directly at ICAR-CIFT office"
Letter, DD & Samples send to "The Director, ICAR-CIFT, Willingdon Island, Cochin, Kerala-682029 "