Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Directorate of Groundnut Research, Ivnagar Road, P.B.No.5, Junagadh-362001 Gujarat (www.dgr.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Microplate Photometer (ELISA reader)
Make                                   : Thermo Scientific
Model                                  : Multiskan FC Microplate Photometer
Specification   :It is a Filter-based microplate photometer with shaking and incubation upto 50 °C. It has Quartz-halogen lamp (Osram 64222, 6V/10W), interference filter (in the filter wheel), fiber end optics, silicon photodetector, standard filters for 405 nm; 450 nm, and 620 nm.

Click to View• Detection wavelength from 340 to 850nm • Fast reading of both 96- and 384-well plates • Measurement range is 0–6 Abs • Wavelength accuracy is 2 nm

  Working Principles
Click to ViewIt has one-channel optics with a tungsten halogen lamp as the light source, where wavelength selection is made by means of interference filters and the selected wavelength is directed at a moving measurement head by an optic fiber.. During measurement, the light from the lamp passes through an interference filter to an optic fiber. The filter selects from the lamp spectrum the exact wavelength desired and the optic fiber directs the light below the selected well of the plate. After passing through the well, the light is sensed by a photodetector in the measurement head above the well. Blank (air) and dark level measurements are also needed to calculate the absorbances

  Applications
  User Instructions
• Analysis of aflatoxin B1, B2, G1 and G2 1.Minimum of 10 samples should be submitted 2.Provide minimum 100 g sample per analysis

  Contact Us
Contact No. 0285 2673041
Email Id director.dgr@icar.gov.in

  Microplate Photometer (ELISA reader) Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Aflatoxin B1 analysis from groundnut kernel
3000
2000
2000
per sample
Aflatoxin total (B1+ B2+ G1+ G2) analysis from groundnut kernel
3000
2000
2000
per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""ICAR-Directorate of Groundnut Research, Junagadh OR Electronic transfer to ICAR-Directorate of Groundnut Research, Junagadh (ACC No: 10283132519 IFSC Code: SBIN0003251)""
Letter, DD & Samples send to ""Director, ICAR-Directorate of Groundnut Research, PB 5, Junagadh, Gujarat, India-362001""