Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
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Instrument Details
Instrument/Facility Name: Microtome Sectioning and Microscope Photography Equipment
Make                                   : Leica (Microtome) Leitz Diaplan (Compound Microscope)
Model                                  : RM2145 (Microtome) DM2500 (Compound Microscope)
Specification   :a. Micrtotome: The Leica RM 2145 is a semi-motorized rotary microtome with sectioning thickness of 5 µm to 60 µm. It has 27 mm ±1 mm via step motor total horizontal specimen feed and 70 mm vertical specimen stroke. Instrument is provided with 0 - 100 µm, in 5 µm increments specimen retraction mode. b. Compound microscope: Leitz diaplan microscope consists of lamphousing 103 Z, coarse and fine focus controls, binocular observation tube, PL fluotar objectives, field diaphragm, aperture diaphragm, stage rotation button, lamp holder mount cover.

Click to ViewMicrtotome: The Leica RM 2145 is a semi-motorized rotary microtome. The specimen feed system with zero-backlash and maintenance-free cross roller guides and the step motor operated coarse feed system are located in a dustproof plastic housing. Sectioning is performed by manually rotating the extremely smooth-running handwheel. The handwheel is lockable in any position via a mechanical quick locking system. All settings, LED indications and control functions are centralized in a separate control panel. Control buttons and indications are logically arranged in functional groups. The specimen retraction can be set in different thicknesses and can also be entirely switched off. The retraction phase is visually indicated. The motorized coarse feed operates at two speeds. In the trimming mode, the coarse feed buttons have a TRIM-STEP function. The instrument offers two different manual sectioning modes: conventional sectioning by complete handwheel rotations and sectioning in the ‘Rocking Mode’, where the handwheel only needs to be moved back and forth over a small distance in each direction. The instrument also has a number of special functions, such as the programmable horizontal specimen stop function, and the sectioning and trimming program. Compound microscope: The Diaplan was a model introduced by Leitz. Diaplan DM2500 consists of 10x, 25x, 40x and 100x with oil immersion objectives and phase contrast facility for live observation of specimen. The compound microscope is fitted with camera connected to PC for capturing images through Leica Application Suite Version 4.3.0 (LAS) software.

  Working Principles
Click to ViewMicrotome use steel blades to prepare sections of plant tissues for light microscopy histology. Microtome is a sectioning instrument that allows the cutting of extremely thin slices of a material known as section. Microtome is used in microscopy, allowing for the preparation of sample for observation under transmitted light or electrons radiation. A compound microscope magnifies the size of the object by a complex system of lens arrangement. It has a series of two lenses; (i) the objective lens close to the object to be observed and (ii) the ocular lens or eyepiece, through which the image is viewed by eye. Light from a light source (mirror or electric lamp) passes through a thin transparent object. The objective lens produces a magnified ‘real image’ first image) of the object. This image is again magnified by the ocular lens (eyepiece) to obtain a magnified ‘virtual image’ (final image), which can be seen by eye through the eyepiece. As light passes directly from the source to the eye through the two lenses, the field of vision is brightly illuminated. That is why; it is a bright-field microscope.

  Applications
  User Instructions
Microtome: Histological study of various tissues. Microscope: Observing cells for mitotic activity and identification of cellular storage components. Follow the instructions as per the User manual.Prior confirmation may be obtained from the Institute before taking DD / sending samples.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  Microtome Sectioning and Microscope Photography Equipment Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Microtome Sectioning and Microscope Photography
10000
4500
5000
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod"
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"