Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur-208024 Uttar Pradesh (www.iipr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Molecular Imager Systems
Make                                   : Bio-Rad
Model                                  : Pharox FX Plus system
Specification   :used for the sensitive detection and analysis of DNA, RNA, or protein samples in gels, blots, or microplates. Detect a wide range of fluorophores with optional 488 nm and 635 nm external lasers

Click to ViewInstrument Specifications Dynamic range 5 orders of magnitude (Accurately quantitates 32P, 33P, 35S, 14C, and 3H ) Linearity r2 > 0.99 Uniformity ±5% over entire scan area Scan resolution 800, 200, 100, and 50 µm (user selectable) Scan time 20 x 25 cm area: 8.5 min at 100 µm, 15 min at 50 µm 35 x 43 cm area: 8.5 min at 200 µm, 17 min at 100 µm Digital resolution 16-bit (65,536 gray scale) Excitation source 25 mW 532 nm (green) diode-pumped solid-state laser 10 mW 635 nm diode laser

  Working Principles
Click to ViewIn this machine, optimized excitation/emission filter combinations deliver optimal signal-to-noise and thus exceptional sensitivity. Flexibility in the choice of filters, together with software control, allows extensive user customization. The Pharos FX Plus imagers detect photons with a variable-gain photomultiplier tube (PMT) assembly. The variable PMT gain is software controlled, and can be used to boost imaging sensitivity for enhanced detection of low-abundance proteins or small quantities of fluorescently labeled nucleotides.

  Applications
  User Instructions
 Proteomic Applications  Genomic Applications etc.  No gels thicker than 8 mm  Gels blot or membrane should be wet  Use sample holders to keep sample from moving during scan

  Contact Us
Contact No. 0512-2580986
Email Id diriipr.icar@gmail.com

  Molecular Imager Systems Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""