Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Sheep & Wool Research Institute, Avikanagar, via Jaipur-304501, Rajasthan (www.cswri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Motility Analyzer
Make                                   : Hamilton Thorne Research
Model                                  : HTM-IVOS version 12
Specification   :• Integrated optics, Automated internal specimen stage,Real-time quality control, Multispecies sperm analysis compatibility,Summary of sperm analysis such as count, concentration , mean values and bar chart distribution

Click to View• Heated stage: Temp. Control: Room Temp. To 45 ˚C • Temp step size: 0.1˚C and Temp. Stability: 0.5 ˚C • Specimen chamber: Makler chamber: depth 10 µm. Internal Optical system: imaging device: CCD • Objective: 10X standard • Image type: phase contrast Digital image acquisition: • Frames: min 5 Max. 100 • Frame rate: 60, 30 per sec • Illumination: Stroboscopic light source • Photometer: scale on screen • Intensity: computer controlled • Fields: 1-20 • Manual/automated field selection Analyzing system • Image resolution: 640 x 480 • Control: mouse, keyboard • Software: on hard disk • Analysis set: 7 user-defined • IVOS computer system • Operating system: Windows MAC • RAM: 500 MB • Monitor: 20” Black white • Hard Drive: • Floppy drive: 1.44 MB 3.5’’

  Working Principles
Click to ViewThe IVOS version 12 Motility Analyzer operates as a cell motion analyzer and a computer. Integrated hardware components include an internal phase-contrast optics system with stroboscopic illumination, an internal automated heated specimen stage, an image digitizer and a computer. Semen samples following dilution at 25 million sperm per ml in normal saline is loaded onto a Makler counting chamber and placed on the stage of the phase contrast microscope and inserted in to the machine. Then with the help of computer-operated digital camera several frames of video images are captured, analyzed by the inbuilt software and average values of different motility parameters are displayed on the result screen.

  Applications
  User Instructions
Analysis of sperm motility and kinematics. Besides, the apparatus can analyse sperm morphology with the help of optional Morphometry analysis software (Real Time Morphology).As per manual

  Contact Us
Contact No. 01437 220165, 9460549384
Email Id raghvendar@gmail.com

  Motility Analyzer Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
200/- per
100/- per
100/- per

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT -CSWRI, Avikanagar payable at SBI, MalpuraTonk Rajasthan"
Letter, DD & Samples send to "Dr Raghvendar Singh, Head Division of Animal Physiology and Biochemistry, ICAR-CSWRI, Avikanagar, Via- Jaipur-304501 "