Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Abiotic Stress Management, Malegaon, Baramati-413115, Pune, Maharashtra (www.niam.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Multi-mode Microplate Reader
Make                                   : BMG-Labtech
Model                                  : CLARIOstar
Specification   :Detection Modes: 1. Fluorescence intensity 2. Fluorescence polarization 3. AlphaScreen®/AlphaLISA®/AlphaPlexTM 4. Luminescence (flash and glow) - incl. BRET 5. Time-resolved fluorescence 6. TR-FRET 7. UV/Vis absorbance spectra; Measurement Modes: Top and bottom reading, Endpoint and kinetic, Sequential multi-excitation, Sequential multi-emission, Spectral scanning (fluorescence, luminescence, absorbance), Ratiometric measurements & Well scanning; Spectral Range: Filters - 240 - 750 nm for FI, FP, TRF 240 - 750 nm for LUM, LVF Monochromator :- 320 - 850 nm for FI 320 - 750 nm for LUM, Linear Variable Dichroic :- 340 - 740 nm for FI, LUM, Spectrometer:- 220 - 1000 nm for ABS; LVis Plate (Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples)

Click to ViewThe CLARIOstar is a multi-mode, high-performance microplate reader which can be used for various applications in the different detection modes. The detection mode includes LVF Monochromators, Spectrometer and Filters. It performs non-isotopic detection including UV/Vis Absorbance, Fluorescence Intensity and Luminescence (flash and glow). It supports applications such as DNA, RNA, and protein quantification, Cell based assays, Enzyme activity and kinetic assays, Reporter gene assays, Protein-protein interactions and Molecular binding assays.

  Working Principles
Click to ViewThe CLARIOstar is a multi-mode reader based on Monochromators, Spectrometer and Filters detection mode. Its measurement mode includes Top and Bottom reading, Endpoint and Kinetic measurements, Spectral Scanning (Fluorescence, Luminescence and Absorbance) and well scanning. Instrument having 2 built-in reagent injectors with reagent back flushing with individual injection volumes for each well 3 to 500 μL and variable injection speed up to 420 μl.

  Applications
  User Instructions
DNA, RNA, and protein quantification, Cell based assays, Enzyme activity and kinetic assays, Reporter gene assays, Protein-protein interactions and Molecular binding assaysAs per user mannual

  Contact Us
Contact No. 02112-254057/8
Email Id director.niasm@icar.gov.in

  Multi-mode Microplate Reader Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Samples
350
200
100
+GST as applicable.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit NIASM"
Letter, DD & Samples send to "ICAR-National Institute of Abiotic Stress Management, Malegaon Khurd, Baramati-413115, Pune, MH"