Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Research Centre on Meat, Chengicherla, P.B.No.19, Boduppal P.O., Hyderabad-500092, Telangana (www.nrcmeat.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: OFFGEL Fractionator
Make                                   : Agilent Technologies
Model                                  : 3100 OFFGEL Fractionator
Specification   :pI-based OFFGEL fractionation with in-solution recovery for easy transfer to downstream methods such as LC/MS. • Reproducible pI-based OFFGEL fractionation down to 0.1 pH resolution depending on the consumable kit used. • Preconfigured methods for OFFGEL and traditional in-gel IEF protein and peptide fractionation. • pI obtained acts as an additional validation parameter. • No MS-incompatible additives used in fractionation buffer • Fractionation of up to 16 samples in parallel on 2 trays, accommodating 8 samples each.

Click to ViewOFFGEL electrophoresis uses multi-compartment chamber on top of an IPG strip which provides the desired pH gradient and solubilized proteins migrate through the strip until they reach their isoelectric point (pI) at a given compartment and then return into solution. Protein fractions are separated in Agilent 3100 OFFGEL fractionator high resolution mode (24 fractions per 24 cm, 3-10 IPG strip) with the Agilent’s proprietary starter kit

  Working Principles
Click to ViewSeparates purified proteins into 24 fractions in liquid form on a 24 cm immobilised pH Gradient (IPG) strip based on their iso-electric point (pH range 4-7 or 3-10)

  Applications
  User Instructions
Used for separation of purified proteins based on their iso-electric pointAs per user manual

  Contact Us
Contact No. 040 29801672
Email Id nrcmeat_director@yahoo.co.in

  OFFGEL Fractionator Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
OFFGel Fractionation
8000
8000
Per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit, NRC on Meat payable at Hyderabad"
Letter, DD & Samples send to ""