Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur-208024 Uttar Pradesh (www.iipr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: PCR Thermal Cycler
Make                                   : G-Storm
Model                                  : GS0004M
Specification   :Simple way of copying specific DNA fragments from minute quantities of source DNA material

Click to ViewThermal Blocks Temperature Control: 4°C - 99°C with simulated volume dependent control algorithm Sample Volume Range 5-150μl Sample Homogeneity +/- 0.4°C Heating Up to 3°C per second Cooling Up to 3°C per second Sample Overshoot < 1°C Gradient Temperature Range 30°C - 80°C Maximum/minimum Gradient Span 30°C / 1°C Sample Accuracy +/- 0.4°C (20-99°C), +/- 1°C (4-20°C) Special feature: Touchdown: It is an advanced technique used to reduce non-specific binding. With the G-Storm thermal cycler, Touchdown is provided as a one-step command for easy implementation in the programming sequence.

  Working Principles
Click to ViewAs the name implies, it is a chain reaction, a small fragment of the DNA section of interest needs to be identified which serves as the template for producing the primers that initiate the reaction. One DNA molecule is used to produce two copies, then four, then eight and so forth. This continuous doubling is accomplished by specific proteins known as polymerases, enzymes that are able to string together individual DNA building blocks to form long molecular strands. To do their job polymerases require a supply of DNA building blocks, i.e., the nucleotides consisting of the four bases adenine (A), thymine (T), cytosine (C) and guanine (G). They also need a small fragment of DNA, known as the primer, to which they attach the building blocks as well as a longer DNA molecule to serve as a template for constructing the new strand. If these three ingredients are supplied, the enzymes will construct exact copies of the templates.

  Applications
  User Instructions
 Selective DNA isolation  Forensic applications  Medical applications  Amplification and quantification of DNA  Infectious disease applications  Research applications  96 sample can be used in a single gradient blocks.  The thermal blocks, inner side of heated lids and reaction vessels quickly attain temperatures of greater than 50°C. Do not touch –Risk of Burns!  Do not use any materials (plates, sealings, foils, mats), which are not sufficiently temperature-stable (up to 120 °C).  96 well gradient block: For 96 well plates or 96x 0.2 ml tubes  Reaction vessels should be filled outside the cycler so that no fluids penetrate the instrument.

  Contact Us
Contact No. 0512-2580986
Email Id diriipr.icar@gmail.com

  PCR Thermal Cycler Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""