Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Marine Fisheries Research Institute, P.B.No.1603, Ernakulam North P.O., Abraham Madamakkal Road, Kochi-682018 Kerala (www.cmfri.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: PCR Thermocycler; White spot virus screening using Nested PCR method
Make                                   : Biorad
Model                                  : T100TM THERMAL CYCLER
Specification   :PCR Thermocycler; White spot virus screening using Nested PCR method

Click to ViewThermal cycler with capacity 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate, maximum ramp rate, 40C, average ramp rate, 2.50C, temperature range 4-1000C, gradient range 30-1000C, easy to programme using a large color touch screen, thermal gradient allows PCR optimization using a unique thermal gradient

  Working Principles
Click to ViewThe device consists of a thermal block with holes where tubes with reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete steps which are pre-programmed. Using the peltier effect, heating and cooling of the block holding the PCR tubes are carried out simply by reversing the electric current. Reaction tubes which are thin walled permit favourable thermal conductivity to allow for quick thermal equilibration. Heated lids prevent condensation at the top of the reaction tube.

  Applications
  User Instructions
Amplification of DNA for genetic and genomic studiesTo avoid contamination please use pipettes with plugged tips specifically for PCR, keep specific areas for PCR reaction preparation and PCR product analysis

  Contact Us
Contact No. 9496280584
Email Id vgcochin@hotmail.com

  PCR Thermocycler; White spot virus screening using Nested PCR method Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Polymerase Chain Reaction
1000
1000
1000
Price per sample. Additional Rs 100/-towards postal charges if result are to be posted.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, ICAR-CMFRI, Cochin OR the payment can be made directly at ICAR-CMFRI office". Letter, DD & Samples send to "The Director, ICAR-CMFRI, Ernakulam North PO, Kochi-682018, Kerala"
Letter, DD & Samples send to "The Director, ICAR-CMFRI, Ernakulam North P.O., Kochi, 682018"