Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute for Arid Horticulture, Sri Ganganagar Highway, Beechwal Industrial, Area Post Off. Bikaner-334006 Rajasthan (https://ciah.icar.gov.in)
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Instrument Details
Instrument/Facility Name: Polymerase Chain Reaction (PCR) – 96 well
Make                                   : Thermo Electron Corporation
Model                                  : Px2 thermal cycler
Specification   :The PCR is a laboratory instruments used for amplification of DNA fragments in desired quantity for molecular analysis of organisms including plants. The specifications of available PCR machine are as follow: Block uniformity ± 0.3°C within 30 seconds. Ramping speed is up to 3°C per second heating, 2°C per second cooling. Precise control is 0.1°C. Block temperature range 4- 99°C. Power consumption is 550 W. Weight 9 kg. Size (w x d x h) 240mm x 390mm x 280mm. Display 1/4 VGA (320 x 240) Ports RS 232. Programs is 99 Blocks with 96 well of 0.2ml (standard and gradient), 96 well 0.5ml (standard and gradient). Easy to perform the setup.

Click to ViewThe PCR is a laboratory instruments used for amplification of DNA fragments in desired quantity for molecular analysis of organisms including plants. The specifications of available PCR machine are as follow: Block uniformity ± 0.3°C within 30 seconds. Ramping speed is up to 3°C per second heating, 2°C per second cooling. Precise control is 0.1°C. Block temperature range 4- 99°C. Power consumption is 550 W. Weight 9 kg. Size (w x d x h) 240mm x 390mm x 280mm. Display 1/4 VGA (320 x 240) Ports RS 232. Programs is 99 Blocks with 96 well of 0.2ml (standard and gradient), 96 well 0.5ml (standard and gradient). Easy to perform the setup.

  Working Principles
Click to ViewAs the name implies, it is a chain reaction, a small fragment of the DNA section of interest needs to be identified which serves as the template for producing the primers that initiate the reaction. One DNA molecule is used to produce two copies, then four, then eight and so forth. This continuous doubling is accomplished by specific proteins known as polymerases, enzymes that are able to string together individual DNA building blocks to form long molecular strands. To do their job polymerases require a supply of DNA building blocks, i.e., the nucleotides consisting of the four bases adenine (A), thymine (T), cytosine (C) and guanine (G). They also need a small fragment of DNA, known as the primer, to which they attach the building blocks as well as a longer DNA molecule to serve as a template for constructing the new strand. If these three ingredients are supplied, the enzymes will construct exact copies of the templates. It is then possible to clone DNA whose sequence is unknown. This is one of the method's major advantages. Genes are commonly flanked by similar stretches of nucleic acid. Once identified, these patterns can be used to clone unknown genes - a method that has supplanted the technique of molecular cloning in which DNA fragments are tediously copied in bacteria or other host organisms. With the PCR method this goal can be achieved faster, more easily and above all in vitro, i.e., in the test-tube. Moreover, known sections of long DNA molecules, e.g. of chromosomes, can be used in PCR to scout further into unknown areas.

  Applications
  User Instructions
Thermal cycler is a technologically advanced laboratory apparatus that is useful for various experiments. This device is also known as a PCR machine or DNA amplifier because it helps in amplifying the segments of DNA via the polymerase chain reaction. Carrying out the polymerase chain reaction of DNA for scientific experiments Helping in maintaining the temperature during biochemical reactions such as DNA cloning for sequencing, DNA amplifying, and DNA-based phylogeny Heating and cooling the DNA samples for genotyping Carrying out genetic tests that are helpful in clinical diagnosis of certain diseases Creating thermal gradients in order to find the optimal PCR conditions in experiments like primer annealing.Kindly ensure that all the PCR components must be on ice during entire process. Template (DNA/cDNA) should be purified. Kindly make the 20-50 ng/ul dilution of template in Tris-HCL or T10E1 or Nuclease-free water. Primer(s) should be diluted as 10 pcomole/µl concentration.

  Contact Us
Contact No. 9540939595
Email Id chetram.nbpgr@gmail.com

  Polymerase Chain Reaction (PCR) – 96 well Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
PCR
500
300
300
Primer should be supplied with sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT - A/C, CIAH Bikaner"
Letter, DD & Samples send to "Director, ICAR-CIAH, 10 Km milestone Sriganganagar Highway, Beechwal Bikaner Rajasthan-334006"