Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Coastal Agricultural Research Institute, Ela, Old Goa, Distt. North Goa, Goa-403402 (https://ccari.icar.gov.in/)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Pulse Field Gel Electrophoresis (PFGE)
Make                                   : Bio-Rad Laboratories, Inc.
Model                                  : CHEF-DR® II Pulsed Field Electrophoresis Systems
Specification   :Pulsed field electrophoresis is a technique for resolving chromosome-sized DNA molecules. By alternating the electric field between spatially distinct pairs of electrodes, megabase (mb) sized molecules are able to reorient and move at different speeds through the pores in an agarose gel. CHEF-DR II Specifications includes- Drive Module, control module, mini chillar, Electrophoresis cell and accessories to prepare agarose gel and run the bacterial genomes

Click to ViewPulsed field electrophoresis works by alternating the electric field between spatially distinct pairs of electrodes, DNA molecules are able to reorient and move at different speeds through the pores in an agarose gel.

  Working Principles
Click to ViewThe CHEF-DR II system is based on CHEF (Clamped Homogeneous Electric Fields) technology.

  Applications
  User Instructions
PFGE in microbiology is a standard method which is used for typing of bacteria based on separation of large DNA fragments. Strain diversity (insertions, deletions & rearrangements) give rise to changes in DNA fragments which can be detected by PFGE profile of Bacterial DNA fragments Genotyping of E coli/Salmonella strains from animal origin protocols are standardised in this laboratory. User should bring the isolates, RE enzymes, buffers, PFGE consumables and can avail the PFGE facility to run the isolates

  Contact Us
Contact No. 0832- 2284678/Intercomm-312
Email Id shivasharanappa.n@icar.gov.in

  Pulse Field Gel Electrophoresis (PFGE) Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
PFGE
6000/sampl
3000/sampl
3000/sampl
This is in addition to consumables required for running the protocol

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT: CCARI, GOA Payable at goa"
Letter, DD & Samples send to "To, The Director, ICAR-Central Coastal Agricultural Research Institute, Ela, Old Goa - 403 402, Goa "