Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), ICAR Campus, Ramagondanahalli, Yelahanka, Bengaluru-560064 Karnataka (www.nivedi.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Pulsefield Gel Electrophoresis
Make                                   : Biorad Pacific Ltd , Hongkong
Model                                  : CHEF-DR® III Variable Angle System
Specification   :trophoresis cell dimension: 11.4 x 44.2 x 50.3 cm, horizontal format

Click to View• Electrophoresis cell dimension: 11.4 x 44.2 x 50.3 cm, horizontal format • Power module dimension: --> Construction: Aluminum chassis --> Weight: 10 kg • Maximum amps: 0.5 amperes • Allowable voltage gradients: 0.6–9 V/cm, in 0.1 V/cm increments

  Working Principles
Click to ViewDNA is pulled through a PFGE gel due to electric charge, similar to the standard electrophoresis run. In principle, the apparatus itself is essentially the same as a standard electrophoresis unit. Similar to standard electrophoresis, there are electrodes that allow the electric charge to pass through the chamber. In PFGE however, these electrodes surround the gel and are not all active at once. Activating only certain electrodes is what allows the electric current to be modulated at specific angles. While a standard gel is usually only active for a couple hours, PFGE runs can take days. To keep the buffer temperature down, it is run through tubing and through a cooler before being fed back into the chamber.

  Applications
  User Instructions
Fast and high-resolution separations of DNA molecules ranging from 200 kb to >6 Mb --> Top down and bottom up mapping --> Electrophoretic karyotyping --> Analysis of tumor cell DNA rearrangements --> Mammalian DNA analysis --> Testing for bacterial, yeast, and parasite strain homogeneity.• User has to pre-run the instrument 15-20 minutes prior to actual run for calibration. • User has to use the deionized distilled water for the electrophoresis. • Due care has to be followed for constant supply of the current without any voltaze fluctuation. • When the parameters are set, start the program by pressing PAUSE/START RUN. When the program is in progress, the left panel display will show the time remaining (hours) in the current Block with RUN TIME lit, and the right panel display will show the actual current (milliamps) with ACTUAL CURRENT and PAUSE/START RUN lit. After the program is started, it is not possible to edit any of the run parameters. • The program in progress may be manually terminated by holding down PAUSE/START RUN for 3– 4 seconds. A program can be terminated only while it is in the run mode; it cannot be terminated in PAUSE. When the program is terminated two beeps will sound, and the right display will show OFF. Pressing PAUSE/START RUN again will start the program from the beginning. • When the program terminates under the timer control, the PAUSE/START RUN light will go off, it will sound two beeps per second for 5 seconds, and the right display will show OFF. The run timers will be reset and all parameters will be retained. The run parameters may be used again as is, or further modified, and the program may be started again by pressing PAUSE/START RUN

  Contact Us
Contact No. 080-23093111
Email Id director.nivedi@icar.gov.in

  Pulsefield Gel Electrophoresis Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
2500
1000
2500

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""