Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Inland Fisheries Research Institute, PO Monirampare, Barrackpore, Kolkata-700120 West Bengal (www.cifri.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: QUANTITATIVE PCR
Make                                   : BIO-RAD
Model                                  : CFX Connect optics module
Specification   :TEMPERATURE RANGE:: 30–100°C MAXIMUM HEATING RATE: 5°C/sec (Maximum) MAXIMUM COOLING RATE: 5°C/sec (Maximum) , CFX Connect optical reaction module excite fluorophores (SYBR®Green I, FAM etc.) by 3 filtered LEDs (450–535 nm) and detect emission by 3 photodiodes (515–580 nm) with the sensitivity of 1 copy. Excites and detects fluorophores in three channels and uses multiple data acquisition scan modes to collect fluorescence data during a run.CFX Manager software to calculate end data.

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  Working Principles
Click to ViewThe instrument Real-Time PCR, also known as quantitative polymerase chain reaction (qPCR) runs on the principles of polymerase chain reaction (PCR) where amount of product amplified during each cycle is linked to fluorescence intensity using a non-specific fluorescent reporter, SYBR green molecule for detection and quantitative measurement. A threshold level of fluorescence is set above the background within the linear phase of amplification for all the plots. The cycle number at which an amplification plot crosses the threshold fluorescence level is called the “Ct” or threshold cycle. The Ct value can be directly correlated to the starting target concentration of the sample. The greater the amount of initial DNA template in the sample, the earlier the Ct value for that sample. All C t data were transported to MS-Excel for further processing and final result was represented as normalized expression (2 -ΔCt ) or comparative expression (2 -ΔΔCt ).

  Applications
  User Instructions
Relative quantification of gene of interest in biological samples cDNA/DNA samples of along with primers (real time) of gene of interest and reference gene are to be sent in dry ice situation or-20° C condition.

  Contact Us
Contact No. 033 25920177
Email Id director.cifri@gmail.com

  QUANTITATIVE PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Dr. Md. Aftabuddin
Working condition

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit, CIFRI, Barrackpore"
Letter, DD & Samples send to "Director, ICAR- Central Inland Fisheries Research Institute Barrackpore, Kolkata-700120, W.B. Telefax: 033 25920177 E- mail: director.cifri@gmail.com"