Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Institute of Fisheries Technology, CIFT Junction Matsyapuri PO, Cochin-682029, Kerala (www.cift.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real Time PCR
Make                                   : Thermofischer scientific, USA
Model                                  : 7500 Realtime PCR system
Specification   :Capacity: 1 x 96-well plate (Fast), 12 x 8-tube strips; Reaction Volume Range: 10-30 µl

Click to ViewSensitivity: (Concentric Nebuliser) better than 109 counts per seconds for 1 ppm of In Quantification Limit: Better than 1 ppt. Dynamic Range: Linear for 12 decades, i.e. 1012 Dark Noise: Less than 0.2 counts per second. Signal Stability: Better than < 0.5 % RSD over 30 minutes Better than <1 % RSD over 1 hour. Mass Stability: <10 ppm over one hour < 20 ppm over 10 hours. Scan Speed: (Magnetic) 1 to 240 amu in less than 70 ms. Scan Speed: (Electric) less than 1 ms / jump irrespective of mass range. Oxides & Doubly charged Ions: BaO+ /Ba+ less than <0 .002 % Ba2+ / Ba+ less than <0.02 % Radio Frequency Generator: 27 MHz with 2 KW RF Power with automatic Reflected power matching network and fully protected against over load and heating. RF section air - cooled and load coil water cooled with its own standalone heat exchanger. Plasma Torch: Full demountable torch with sample tube (ceramic / Sapphire injector) suitable for HF based solutions and additional injector for organic solvents. Computer controlled three Argon Mass Flow Controller for torch and nebuliser. Nebuliser: Concentric Nebulizer for normal acid based solution with flow rate of 50 micro-litre / min and 100 micro-litre / min. Suitable Nebuliser for HF based solution with cyclonic spray chamber. Spray Chambers: 1. Dual Cyclone double pass Quartz Spray chamber for high accuracy measurement compatible with Peltier cooling. 2. PFA Spray chamber for ultra trace analysis and HF based solutions. 3. Peristaltic Pump: For constant (regulated) sample flow and drain. Peltier Cooling System: Fully Computer controlled Peltier cooling system for accurate temperature control with quartz / PFA Spray chamber.

  Working Principles
Click to ViewThe 7500 software v2.0 for the 7500 Fast and 7500 Real-time PCR Systems runs on the Windows® XP and Microsoft Vista OS operating systems. Flexible five-color detection systems are easily calibrated for various dyes without requiring the addition of new filter sets. Variable excitation capacity allows greater sensitivity for the greatest range of dyes.A real-time polymerase chainreaction (Real-Time PCR), also known asquantitative polymerase chain reaction(qPCR), theamplification of a targeted DNA moleculeduring the PCR are monitored on a real time.

  Applications
  User Instructions
Provide the type of experiment to be performed on the sample given. Primer and target DNA details and type of the assay should be included. Cycling condition of the assay should be given.

  Contact Us
Contact No. 0484-2412300
Email Id prasadmm@hotmail.com

  Real Time PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Standardization and entire setup (User provides details of primers, dye and template in Pure form)
2000
500
1000
per reaction/per sample
Only Plate set up (User provides primers, dye and template in pure form)
600
200
400
per reaction/per sample

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, ICAR-CIFT, Cochin OR the payment can be made directly at ICAR-CIFT office"
Letter, DD & Samples send to "The Director, ICAR-CIFT, Willingdon Island, Cochin, Kerala-682029 "