Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-National Research Centre on Meat, Chengicherla, P.B.No.19, Boduppal P.O., Hyderabad-500092, Telangana (www.nrcmeat.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real time PCR machine
Make                                   : Bio-Rad
Model                                  : CFX 96
Specification   :Max ramp rate: 5° C/sec Av ramp rate: 3.3° C/sec Peltier heating & cooling Lid temperature: 105° C Range: 0-100° C Accuracy: ± 0.2° of programmed target at 90° C Operational range: 30-100° C Detection: 6 photodiodes Range of emission:450-730nm

Click to ViewThe CFX96 Touch system is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestroâ„¢ Software to easily and intuitively design your experiment and analyze results from a connected computer.

  Working Principles
Click to ViewReal time PCR monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively to detect the concentration of nucleic acid based on Ct values.

  Applications
  User Instructions
Real time PCR for quantification of DNA or RNA in samples.As per user manual

  Contact Us
Contact No. 040 29801672
Email Id nrcmeat_director@yahoo.co.in

  Real time PCR machine Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Polymerase Chain Reaction
2000/96 we
2000
Per 96 well plate without consumables

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR Unit, NRC on Meat payable at Hyderabad"
Letter, DD & Samples send to ""