Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Plantation Crops Research Institute, Kudlu P.O., Kasaragod-671124, Kerala (www.cpcri.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real time PCR
Make                                   : Applied Biosystems
Model                                  : StepOneâ„¢ Real-Time PCR System
Specification   :The StepOneâ„¢ (48 well) , Three-color Real-Time PCR Systems are remarkably simple to use. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced real-time PCR researchers. Despite their simplicity, the advanced technology will deliver you precise and reliable results every time.

Click to ViewThe StepOne™ Systems bring advanced real-time PCR technology to a new level of accessibility—even for first-time users. It measures amplification as it occurs, cycle by cycle, allowing for precise and quantitative measurements during the exponential phase of PCR. Beginning at the homepage, you can navigate seamlessly through all aspects of the real-time PCR process including sample and reaction setup, thermal cycling, and fluorescent detection. Focused application software analyzes and interprets experimental results. The system provides 2-fold discrimination with 99.7% confidence, along with 9 logs of dynamic range. Highly sensitive, these systems detect 10 copies of RNase P in a 30-μL reaction. Additionally, for laboratories with the StepOne ™ System that require greater throughput, an upgrade kit is available. The StepOne™ Systems software contains unique features not available in other real-time PCR instruments. For example, data can be analyzed from multiple perspectives in the Multiple Plots view. The software constructs four-plot, side-by-side views of all data aspects including theamplification plot, standard curve, multi-component data plots, and raw data. It also displays this data next to the plate layout for easier analysis.

  Working Principles
Click to ViewApplied Biosystems has developed two reagent types (chemistries) that can be used to detect PCR products on the Applied Biosystems StepOne ™ Real-Time PCR Systems: • SYBR ® Green reagents (using) • TaqMan® reagents The SYBR Green reagents use the SYBR Green I dye to detect PCR products by binding to double-stranded DNA formed during the PCR. Here is how it works: • Step 1 When added to a sample, SYBR Green I dye immediately binds to all double-stranded DNA. • Step 2 During the PCR, AmpliTaq Gold® DNA Polymerase amplifies the target, which creates the PCR product, or “amplicon.” The double-stranded DNA is denatured to single-stranded molecules and the SYBR Green I dye is released. • Step 3 The primers anneal to the single-stranded DNA and the Amplitaq Gold DNA Polymerase amplifies the target, creating more double stranded DNA. As the PCR progresses, more amplicon is created. • Step 4 The SYBR Green I dye then binds to each new copy of double-stranded DNA that is generated during each PCR cycle. Since the SYBR Green I dye binds to all double-stranded DNA, the result is an increase in fluorescence intensityproportional to the quantity of double-stranded PCR product produced.

  Applications
  User Instructions
The StepOne ™ Systems support any real-time PCR application. Predesigned or custom assays exist for the following applications: • Gene expression profiling • Gene detection • MicroRNA expression • Mutation scanning • HRM analysisFollow user manual instructions.

  Contact Us
Contact No. 04994232090
Email Id director.cpcri@icar.gov.in

  Real time PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Real time PCR
6000
2500
3000
Prior confirmation may be obtained from the Institute before taking DD / sending samples.

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Unit-CPCRI Kasaragod"
Letter, DD & Samples send to "The Director, ICAR-CPCRI, Kudlu P.O Kasaragod - 671 124. Kerala"