Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Directorate of Coldwater Fisheries Research, Anusandhan Bhavan, Industrial Area, Bhimtal-263136 Uttarakhand (www.dcfr.res.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real time PCR
Make                                   : Bio Rad
Model                                  : CFX 96 Real time PCR
Specification   :Detects 1 copy of target sequence in human genomic DNA with Multiplex analysis Up to 5 targets per well with total capacity og 96 well

Click to ViewThe CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestroâ„¢ Software to easily and intuitively design your experiment and analyze results from a connected computer

  Working Principles
Click to ViewQuantitative reverse transcription PCR (RT-qPCR) is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction. RT-qPCR is used in a variety of applications including gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research.

  Applications
  User Instructions
RT-qPCR is used in a variety of applications including gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research.user can follow link given herewith for detail instruction about sample collection, transportation etc. http://www.bio-rad.com/webroot/web/pdf/lsr/literature/10010424.pdf

  Contact Us
Contact No. 5942 247280
Email Id shivafcri@gmail.com

  Real time PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
600
500
500
RATES ARE ANALYSIS OF PER WELL

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR UNIT DCFR BHIMTAL"
Letter, DD & Samples send to ""