Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Directorate of Groundnut Research, Ivnagar Road, P.B.No.5, Junagadh-362001 Gujarat (www.dgr.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real time PCR
Make                                   : Applied Biosystems
Model                                  : -
Specification   :The Real time PCR system use fluorescent-based polymerase chain reaction to provide quantitative detection of target nucleic acid sequences using real-time analysis, qualitative detection of targets using post-PCR analysis and qualitative analysis of the PCR product (achieved by melt curve analysis that occurs post-PCR).

Click to ViewThe StepOneâ„¢ Real-Time PCR System is a 48-well, low-throughput robust LED based 3-color optical recording instrument for genomic research. It offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation. Simple and flexible instrument set-up and usage.

  Working Principles
Click to ViewThe instrument illuminates all wells of the reaction plate, exciting the fluorophores in each reaction, and collects the residual fluorescence emitted from the wells of the reaction plate. The resulting image collected by the device consists only of light that corresponds to the range of emission wavelengths and further assembles a digital representation of the residual fluorescence collected over a fixed time interval.

  Applications
  User Instructions
SNP genotyping, gene expression analysis, microRNA expression, translocation analysis, gene detection, viral load analysis, quantitative detection of target nucleic acid sequences (targets) using real-time analysis. qualitative detection of targets using post-PCR (endpoint) analysis and qualitative analysis of the PCR Users are advised to send high quality cDNA and DNA along with suitable primers (minimum 48 well)

  Contact Us
Contact No. 0285-2673382 Ext 216
Email Id chandramohan.sangh@icar.gov.in

  Real time PCR Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Gene expression analysis using real time PCR
1000
500
800
The cost mentioned is per sample and excluding GST

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "ICAR-Directorate of Groundnut Research, Junagadh OR Electronic transfer to ICAR-Directorate of Groundnut Research, Junagadh (ACC No: 10283132519 IFSC Code: SBIN0003251)"
Letter, DD & Samples send to "Director, ICAR-Directorate of Groundnut Research, PB 5, Junagadh, Gujarat, India-362001"