Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Central Citrus Research Institute, Post Box No.464, Shankar Nagar P.O., Nagpur-440 010 (www.ccringp.org.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Real-Time thermal Cycler
Make                                   : Applied Biosystems
Model                                  : StepOneTM
Specification   :The StepOneâ„¢ Real-Time PCR System is a 48-well, low-throughput, Real-Time PCR instrument perfect for both first-time and experienced users.

Click to ViewThe StepOneâ„¢ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 3-color optical recording, the StepOneâ„¢ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

  Working Principles
Click to ViewReal-time PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore. The thermal cycler is also able to rapidly heat and chill samples, thereby taking advantage of the physicochemical properties of the nucleic acids and DNA polymerase

  Applications
  User Instructions
Real-time PCR can be used to quantify nucleic acids by two common methods: relative quantification and absolute quantification. Absolute quantification gives the exact number of target DNA molecules by comparison with DNA standards using a calibration curve. It is therefore essential that the PCR of the sample and the standard have the same amplification efficiency. Relative quantification is based on internal reference genes to determine fold-differences in expression of the target gene. The quantification is expressed as the change in expression levels of mRNA interpreted as complementary DNA (cDNA, generated by reverse transcription of mRNA). Relative quantification is easier to carry out as it does not require a calibration curve as the amount of the studied gene is compared to the amount of a control reference geneThe most common problem with Real Time PCR is the contamination of reactions with foreign DNA. To avoid this, Sample should be clean and contamination free.

  Contact Us
Contact No. 0712- 2500249, 2500813 Ext- 139
Email Id ghoshdk@hotmail.com

  Real-Time thermal Cycler Charges
Procedure Name
Industry
University
National LAB/R&D
Remark

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to ""