Indian Council of Agricultural Research
(Agricultural Knowledge Management Information System)


ICAR-Sugarcane Breeding Institute, Veerakeralam, Coimbatore-641007, Tamil Nadu (https://sugarcane.icar.gov.in)
Click Here to See Management Committee
Instrument Details
Instrument/Facility Name: Research (Fluorescent) Microscope with imaging system
Make                                   : Leica Microsystems, Germany
Model                                  : Leica DM LB2 Microscope with DFC280 Digital Camera and Image Analysis software - Leica Q Win V3
Specification   :Light/ Fluorescent Microscope with imaging systems, Image capturing and documentation

Click to ViewOn fluorescent range I3, N2.1, GFP and A filters are available

  Working Principles
Click to ViewMany substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Application of this phenomenon is the basis of fluorescence microscope. In practice, microbes are stained with a fluorescent dye and then illuminated with blue light. The dye absorbs blue light (shorter wavelength) and emits green light (longer wavelength). In a fluorescence microscope, a high intensity mercury arc lamp is used as the light source. It emits white light, which is passed through an ‘exciter filter’. It allows only the blue component of the white light (the white light consists of seven colors, which in the decreasing order of wavelength are violet, indigo, blue, green, yellow, orange and red) to pass through it and blocks out all other color components. The specimen is either previously stained with a fluorescent dye, such as acridine orange NO, acridine yellow or may have fluorescent protein expression system. Thus, the eye perceives the stained portions of the specimen as glowing green object against a jet-black background, whereas the unstained portions of the specimen remain invisible.

  Applications
  User Instructions
All microbial and plant samples can be observed under light microscope. Fluorescent protein expressing and stained samples that are compatible with I3, N2.1, GFP and A filters can be documented. Sample preparation/tissue sectioning should be carried out by the user on their own.

  Contact Us
Contact No. 0422 - 2472621
Email Id director@sugarcane.res.in

  Research (Fluorescent) Microscope with imaging system Charges
Procedure Name
Industry
University
National LAB/R&D
Remark
Sophisticated Analytical Instrument (Research Microscope – Plant Pathology Lab) – Services- reg.
Rs. 100/ho
Rs. 100/ho
Rs. 100/ho

Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of "The Director, Sugarcane Breeding Institute, Coimbatore "
Letter, DD & Samples send to "The Director, Sugarcane Breeding Institute, Coimbatore "